Luo X, Liu Y, Jia R, Shen H, Wang X, Wang M, Zhu D, Chen S, Liu M, Zhao X, Wu Y, Yang Q, Yin Z, Cheng A
Acta Virol. 2018;62(3):330-332. doi: 10.4149/av_2018_227.
Duck Tembusu virus (DTMUV) is a newly emerging enveloped flavivirus. This study shows the ultrastructure of DTMUV using viral purification, negative staining and electron microscopy. Electron microscopic examinations revealed mature DTMUV particles with 50 to 75 nm in diameter and typical enveloped flavivirus structure that consists of the internal nucleocapsid, an inner layer of lipid bilayer and an external layer of E glycoprotein ectodomain. Particles appear to be mostly spherical. In particular, RNA core is deep colored and dense, both capsid and lipid bilayer are clearly visible, the capsid forms regular hexagon, and E glycoprotein ectodomain forms a fringe instead of visible spikes. Thus, this report about the clear ultrastructure of the DTMUV particles will be the major driving forces behind structural biology of DTMUV.
鸭坦布苏病毒（DTMUV）是一种新出现的包膜黄病毒。本研究通过病毒纯化、负染和电子显微镜观察展示了DTMUV的超微结构。电子显微镜检查显示，成熟的DTMUV颗粒直径为50至75纳米，具有典型的包膜黄病毒结构，由内部核衣壳、脂质双层内层和E糖蛋白胞外域外层组成。颗粒大多呈球形。特别是，RNA核心颜色深且致密，衣壳和脂质双层均清晰可见，衣壳形成规则的六边形，E糖蛋白胞外域形成边缘而非可见的刺突。因此，这份关于DTMUV颗粒清晰超微结构的报告将成为DTMUV结构生物学的主要驱动力。
