Galloway D A, Fenoglio C M, McDougall J K
J Virol. 1982 Feb;41(2):686-91. doi: 10.1128/JVI.41.2.686-691.1982.
Human paravertebral ganglia were examined for the presence of specific sequences of viral RNA by in situ cytological hybridization, using as probes individual fragments of herpes simplex virus type 2 DNA. Ganglia were obtained at autopsy from 40 patients, and 14 were positive for herpes simplex virus RNA. Transcripts from the left-hand 30% of the viral genome were detected in all of the herpes simplex virus-positive cases. RNA homologous to other sequences from the L component were present less frequently, and no RNA from the S component was detected. These results argue that specific transcription of the herpes simplex virus genome occurs in latently infected human ganglion cells.
通过原位细胞杂交，使用单纯疱疹病毒2型DNA的单个片段作为探针，检测人椎旁神经节中病毒RNA的特定序列。从40例患者尸检中获取神经节，其中14例单纯疱疹病毒RNA呈阳性。在所有单纯疱疹病毒阳性病例中均检测到病毒基因组左手边30%的转录本。与L成分其他序列同源的RNA出现频率较低，未检测到来自S成分的RNA。这些结果表明，单纯疱疹病毒基因组的特异性转录发生在潜伏感染的人神经节细胞中。
