Hirayama S, Sato F, Oda T, Maeda H
Jpn J Antibiot. 1986 Mar;39(3):815-22.
SMANCS is a conjugate protein of copolymer of styrene-maleic acid [SMA] (molecular weight: 1,500) and an antitumor protein neocarzinostatin [NCS] (molecular weight: 11,700). It has an approximate molecular weight of 15,000. We report here stability of SMANCS in oil and in water, and NCS in water, under various physical conditions such as exposure to heat, UV, pH, and ultrasonic treatment. Then, we carried out an experiment of transfer of SMANCS in lipid contrast medium [lipiodol] (oil phase) to water phase (blood and physiological saline) in vitro. Results are summarized as follows: In aqueous condition, SMANCS is far more stable than NCS against the exposure to heat and UV, though it is inactivated by excessive exposures. SMANCS in an oily medium was found much more stable even at higher temperatures than in the aqueous phase. Both SMANCS and NCS are the most stable at pH 4.9-6.0. SMANCS dissolved in oil transferred to water phase slowly, having T1/10 of 24 hours (in case of lipiodol). This helps maintaining the anticancer effect of the drug in vivo for a long period of time. SMANCS in lipiodol was found to exert its action against cultured tumor cells as in an aqueous solution.
斯马菌素（SMANCS）是苯乙烯 - 马来酸共聚物[SMA]（分子量：1500）与抗肿瘤蛋白新制癌菌素[NCS]（分子量：11700）的结合蛋白。其分子量约为15000。我们在此报告了在诸如受热、紫外线照射、pH值及超声处理等各种物理条件下，SMANCS在油相和水相中的稳定性，以及NCS在水中的稳定性。然后，我们进行了体外实验，观察SMANCS在脂质造影剂[碘油]（油相）中向水相（血液和生理盐水）的转移情况。结果总结如下：在水性条件下，SMANCS在受热和紫外线照射时比NCS稳定得多，不过过度暴露会使其失活。发现油性介质中的SMANCS即使在较高温度下也比水相中稳定得多。SMANCS和NCS在pH 4.9 - 6.0时最稳定。溶解在油中的SMANCS缓慢转移至水相，T1/10为24小时（碘油情况下）。这有助于药物在体内长时间维持抗癌效果。发现碘油中的SMANCS对培养的肿瘤细胞的作用与在水溶液中相同。
