Jaslok Hospital & Research Centre, Mumbai, India.
Inkus Infertility Clinic, Mumbai, India.
Indian J Med Res. 2018 Jun;147(6):552-559. doi: 10.4103/ijmr.IJMR_1186_16.
BACKGROUND & OBJECTIVES: CD9 and CD146 are important adhesion molecules that play a role in the implantation of an embryo. This study was undertaken to correlate the expression of these markers in fertile and infertile women's endometrial stromal cells.
Human endometrial stromal cell culture from endometrial biopsies of fertile (n=50) and infertile females (n=50) was performed and primary cell lines were established. Expression of CD9 and CD146 was studied for all the 100 cell lines with the help of flow cytometry. Gene expression of CD9 and CD146 was performed by real-time polymerase chain reaction.
There was a significant difference in endometrial stromal cells of fertile and infertile females. Flow cytometric results revealed significantly lower expression of CD9 (P=0.0126) and CD146 (P=0.0006) in the infertile endometrial stromal cells as compared to fertile endometrial stromal cells. These results were comparable with real-time data.
INTERPRETATION & CONCLUSIONS: This study showed that endometrial stromal cells from infertile females had lower expression of adhesion molecules, CD9 and CD146. Our findings suggest that CD9 and CD146 may have a role in infertility. Infertile female's endometrial stromal cells have decreased expression of CD9 and CD146 which can be the cause of infertility related to implantation failure.
CD9 和 CD146 是重要的黏附分子，在胚胎植入中发挥作用。本研究旨在探讨这些标志物在生育和不孕女性子宫内膜基质细胞中的表达相关性。
对生育组（n=50）和不孕组（n=50）子宫内膜活检中的人子宫内膜基质细胞进行培养，并建立原代细胞系。采用流式细胞术研究所有 100 个细胞系中 CD9 和 CD146 的表达情况。采用实时聚合酶链反应检测 CD9 和 CD146 的基因表达。
生育和不孕女性的子宫内膜基质细胞存在显著差异。流式细胞术结果显示，与生育的子宫内膜基质细胞相比，不孕的子宫内膜基质细胞中 CD9（P=0.0126）和 CD146（P=0.0006）的表达显著降低。这些结果与实时数据一致。
本研究表明，不孕女性的子宫内膜基质细胞中黏附分子 CD9 和 CD146 的表达较低。我们的发现提示 CD9 和 CD146 可能与不孕有关。不孕女性的子宫内膜基质细胞中 CD9 和 CD146 的表达减少，可能是导致与植入失败相关的不孕的原因。
