Department of General Microbiology, University of Göttingen, Grisebachstrasse 8, 37077 Göttingen, Germany.
Department of General Microbiology, University of Göttingen, Grisebachstrasse 8, 37077 Göttingen, Germany.
Trends Biotechnol. 2019 Jan;37(1):29-37. doi: 10.1016/j.tibtech.2018.08.001. Epub 2018 Aug 29.
Fermentative production of valuable substances is an economically competitive and ecologically sustainable alternative to chemical synthesis, but it is often hampered by detrimental interactions between the heterologous pathway and the host metabolic network. These interactions are diverse and often not understood. However, the growing knowledge about convergently evolved pathways offers an expansive toolbox to engineer novel, hybrid pathways that could circumvent current problems by recruiting the host 'underground metabolism'. Moreover, considering the debate about genetically modified organisms, harnessing the intrinsic capacity of a cell could be promising for food or feed technologies. This opinion article proposes a ubiquitously applicable and technologically simple approach using metabolic rewiring of reversely engineered hybrid pathways.
发酵生产有价值的物质是一种具有经济竞争力和生态可持续性的替代化学合成的方法，但它常常受到异源途径与宿主代谢网络之间有害相互作用的阻碍。这些相互作用是多种多样的，而且往往不被理解。然而，关于趋同进化途径的知识不断增加，为工程设计新型混合途径提供了广泛的工具，这些途径可以通过招募宿主“地下代谢”来规避当前的问题。此外，考虑到关于转基因生物的争论，利用细胞的固有能力可能是有前途的食品或饲料技术。本文提出了一种普遍适用且技术简单的方法，即利用反向工程的混合途径的代谢重排。
