Biomedical Engineering Laboratory, Department of Biomedical Engineering, Federal University of Pernambuco, Recife 50670-901, Brazil.
Department of Virology and Experimental Therapy, Oswaldo Cruz Foundation-FIOCRUZ, Aggeu Magalhães Institute, Recife 50670-420, Brazil.
Biosensors (Basel). 2021 May 16;11(5):157. doi: 10.3390/bios11050157.
Zika virus (ZIKV) is a mosquito-borne infection, predominant in tropical and subtropical regions causing international concern due to the ZIKV disease having been associated with congenital disabilities, especially microcephaly and other congenital abnormalities in the fetus and newborns. Development of strategies that minimize the devastating impact by monitoring and preventing ZIKV transmission through sexual intercourse, especially in pregnant women, since no vaccine is yet available for the prevention or treatment, is critically important. ZIKV infection is generally asymptomatic and cross-reactivity with dengue virus (DENV) is a global concern. An innovative screen-printed electrode (SPE) was developed for amperometric detection of the non-structural protein (NS2B) of ZIKV by exploring the intrinsic redox catalytic activity of Prussian blue (PB), incorporated into a carbon nanotube-polypyrrole composite. Thus, this immunosensor has the advantage of electrochemical detection without adding any redox-probe solution (probe-less detection), allowing a point-of-care diagnosis. It was responsive to serum samples of only ZIKV positive patients and non-responsive to negative ZIKV patients, even if the sample was DENV positive, indicating a possible differential diagnosis between them by NS2B. All samples used here were confirmed by CDC protocols, and immunosensor responses were also checked in the supernatant of C6/36 and in Vero cell cultures infected with ZIKV.
Zika 病毒（ZIKV）是一种蚊媒感染，主要发生在热带和亚热带地区，由于 ZIKV 病与胎儿和新生儿的先天性残疾有关，特别是小头畸形和其他先天性异常，引起了国际关注。由于目前尚无预防或治疗 ZIKV 的疫苗，因此开发通过监测和预防性传播来最大限度减少其破坏性影响的策略至关重要，特别是在孕妇中。ZIKV 感染通常无症状，与登革热病毒（DENV）的交叉反应是一个全球性的关注问题。通过探索普鲁士蓝（PB）的固有氧化还原催化活性，开发了一种用于通过电流检测 Zika 病毒非结构蛋白（NS2B）的丝网印刷电极（SPE），将其掺入到碳纳米管-聚吡咯复合材料中。因此，这种免疫传感器具有电化学检测的优势，无需添加任何氧化还原探针溶液（无探针检测），允许进行即时诊断。它仅对 ZIKV 阳性患者的血清样本有反应，而对 ZIKV 阴性患者无反应，即使样本为 DENV 阳性，表明通过 NS2B 可以对它们进行可能的鉴别诊断。此处使用的所有样本均经 CDC 方案确认，并且还在受 ZIKV 感染的 C6/36 和 Vero 细胞培养物的上清液中检查了免疫传感器的响应。
