Xu Yawei, Wang Junrong, Wang Junling
College of Bioengineering, Jilin Agricultural Science and Technology University, Jilin City 132101, People's Republic of China,
Department of Gynaecology and Obstetrics, China-Japan Union Hospital of Jinlin University, Changchun 130033, People's Republic of China.
Onco Targets Ther. 2018 Aug 21;11:5035-5043. doi: 10.2147/OTT.S170439. eCollection 2018.
The long noncoding RNA X-inactive specific transcript () was reported to play vital roles in tumor progression. In the present study, we determined the regulatory function of in papillary thyroid carcinoma (PTC).
expression was determined in PTC tissues and cell lines by quantitative real-time polymerase chain reaction (PCR) (qRT-PCR). Cellular proliferation, migration, and invasion were measured using the Cell Counting Kit-8 (CCK-8) assay, wound-healing assay, and transwell invasion assay, respectively. Western blotting was used to determine protein expression. The downstream target miRNAs for were identified by luciferase reporter assay and qRT-PCR.
Relative expression of was upregulated in PTC tissues and cell lines. High expression was positively correlated with TNM stage and lymph node metastasis. Function assay demonstrated that knockdown of significantly decreased cell proliferation, migration, and invasion in PTC cells. Moreover, we showed that the effects of on PTC cell progression were mediated by miR-141.
Our results demonstrated that functioned as an oncogene in PTC progression by regulating miR-141, suggesting that might be a promising therapeutic target for PTC treatment.
据报道，长链非编码RNA X染色体失活特异性转录本（）在肿瘤进展中发挥重要作用。在本研究中，我们确定了其在甲状腺乳头状癌（PTC）中的调控功能。
采用定量实时聚合酶链反应（qRT-PCR）检测PTC组织和细胞系中的表达。分别使用细胞计数试剂盒-8（CCK-8）检测、伤口愈合检测和Transwell侵袭检测来测量细胞增殖、迁移和侵袭。采用蛋白质印迹法检测蛋白质表达。通过荧光素酶报告基因检测和qRT-PCR鉴定的下游靶miRNA。
在PTC组织和细胞系中表达上调。高表达与TNM分期和淋巴结转移呈正相关。功能检测表明，敲低可显著降低PTC细胞的增殖、迁移和侵袭。此外，我们发现对PTC细胞进展的影响是由miR-141介导的。
我们的结果表明，通过调控miR-141在PTC进展中发挥癌基因作用，提示可能是PTC治疗的一个有前景的治疗靶点。
