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来自大麻科植物印度大麻（Cannabis sativa L.）花序部分的大麻素对人类病原菌的作用

Cannabinoids from inflorescences fractions of (L.) Blume (Cannabaceae) against human pathogenic bacteria.

作者信息

Napiroon Tiwtawat, Tanruean Keerati, Poolprasert Pisit, Bacher Markus, Balslev Henrik, Poopath Manop, Santimaleeworagun Wichai

机构信息

Department of Biotechnology, Faculty of Science and Technology, Thammasat University, Pathum Thani, Thailand.

Biology program, Faculty of Science and Technology, Pibulsongkram Rajabhat University, Phitsanulok, Thailand.

出版信息

PeerJ. 2021 May 13;9:e11446. doi: 10.7717/peerj.11446. eCollection 2021.

DOI:10.7717/peerj.11446
PMID:34035994
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8126263/
Abstract

BACKGROUND

Cannabinoids; tetrahydrocannabinol (THC), cannabidiol (CBD) and cannabinol (CBN), might show antibacterial activity. is a species in the Cannabaceae that is closely related to through plastome phylogenetic evidence. This species is widely distributed throughout tropical Asia and is used as traditional medicine, particularly for the treatment of infectious diseases. However, no studies on the antibacterial activity of cannabinoid-containing inflorescences extracts are available. Thus, the aim of this study was to determine cannabinoid content and antibacterial activity of inflorescences fractions from native to Thailand.

METHODS

We hypothesized that inflorescences from might display cannabinoids similar to because of their close taxonomic relationship. We extracted the mature inflorescences and infructescence of in three disparate populations from different Thailand floristic regions. Extractions were subsequently partitioned into hydrophilic and lipophilic fractions using distilled water and chloroform. The lipophilic extracts were further fractionated by the column chromatography with gradient elution and analyzed by gas chromatography-mass spectrometry (GC-MS). Characterized cannabinoids were used in bioassays with multidrug-resistance bacteria.

RESULTS

Lipophilic extracts and fractions of inflorescences from all Thailand floristic regions consistently displayed cannabinoids (THC, CBD and CBN) in various quantities. These extracts exhibited inhibitory activity for , , and strains with minimum inhibitory concentration values varying from 31.25 to 125 µg/mL.

CONCLUSION

Our study is the first to report cannabinoid detection in extracts from inflorescences of a species in the Cannabaceae. These extracts and their fractions containing cannabinoids showed pronounced antibacterial activity. The use of analytic methods also demonstrated reproducible cannabinoid extraction.

摘要

背景

大麻素；四氢大麻酚（THC）、大麻二酚（CBD）和大麻酚（CBN）可能具有抗菌活性。通过质体系统发育证据表明，该物种是大麻科中与某物种密切相关的一个物种。该物种广泛分布于热带亚洲，并被用作传统药物，尤其用于治疗传染病。然而，目前尚无关于含大麻素的花序提取物抗菌活性的研究。因此，本研究的目的是确定来自泰国本土该物种花序馏分中的大麻素含量和抗菌活性。

方法

我们假设，由于它们在分类学上的密切关系，该物种的花序可能显示出与某物种相似的大麻素。我们从泰国不同植物区系的三个不同种群中提取了该物种的成熟花序和果实。随后，使用蒸馏水和氯仿将提取物分为亲水性和亲脂性馏分。亲脂性提取物通过梯度洗脱柱色谱进一步分离，并通过气相色谱 - 质谱联用（GC - MS）进行分析。将鉴定出的大麻素用于对多重耐药细菌的生物测定。

结果

来自泰国所有植物区系的花序的亲脂性提取物和馏分始终显示出不同数量的大麻素（THC、CBD和CBN）。这些提取物对某些菌株表现出抑制活性，最小抑菌浓度值在31.25至125μg/mL之间变化。

结论

我们的研究首次报道了在大麻科某物种花序提取物中检测到大麻素。这些含有大麻素的提取物及其馏分显示出显著的抗菌活性。分析方法的使用也证明了大麻素提取的可重复性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d07/8126263/48bc7ccd23e7/peerj-09-11446-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d07/8126263/19500a785c6e/peerj-09-11446-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d07/8126263/b7883f620439/peerj-09-11446-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d07/8126263/8101aef1e2d0/peerj-09-11446-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d07/8126263/48bc7ccd23e7/peerj-09-11446-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d07/8126263/19500a785c6e/peerj-09-11446-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d07/8126263/b7883f620439/peerj-09-11446-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d07/8126263/8101aef1e2d0/peerj-09-11446-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d07/8126263/48bc7ccd23e7/peerj-09-11446-g004.jpg

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