Shinkai K, Mukai M, Akedo H
Cancer Lett. 1986 Jul;32(1):7-13. doi: 10.1016/0304-3835(86)90033-9.
Effect of superoxide radical (O2-) produced extracellularly by hypoxanthine (HX) and xanthine oxidase (XO) on invasive capacity of rat ascites hepatoma cells was studied. Invasive capacity was estimated in vitro by counting the number of tumor cell colonies penetrated underneath cultured mesothelial cell monolayer. When the tumor cells had been treated with non-toxic doses of HX and XO, the formation of penetrated colonies increased with increasing concentrations of XO. This increment was completely inhibited by scavengers of active oxygen radicals, superoxide dismutase (SOD) in combination with catalase (CAT) added simultaneously at the time of HX-XO treatment.
研究了次黄嘌呤（HX）和黄嘌呤氧化酶（XO）在细胞外产生的超氧阴离子自由基（O2-）对大鼠腹水肝癌细胞侵袭能力的影响。通过计数穿透培养的间皮细胞单层下方的肿瘤细胞集落数量来体外评估侵袭能力。当用无毒剂量的HX和XO处理肿瘤细胞时，穿透集落的形成随着XO浓度的增加而增加。在HX-XO处理时同时添加的活性氧自由基清除剂超氧化物歧化酶（SOD）与过氧化氢酶（CAT）可完全抑制这种增加。
