通过在大肠杆菌中克隆揭示的枯草芽孢杆菌中一个簇内精氨酸合成早期酶的基因序列。
Gene sequence encoding early enzymes of arginine synthesis within a cluster in Bacillus subtilis, as revealed by cloning in Escherichia coli.
作者信息
Mountain A, McChesney J, Smith M C, Baumberg S
出版信息
J Bacteriol. 1986 Mar;165(3):1026-8. doi: 10.1128/jb.165.3.1026-1028.1986.
Abstract
From a partial Sau3A gene library of Bacillus subtilis chromosomal DNA in the expression plasmid pRK9, four hybrid plasmids were isolated carrying overlapping segments of the argA-argF-cpa cluster. The complementation patterns within Escherichia coli arginine auxotrophs of these hybrids and deletion derivatives provided the gene order argC-argA-argE-argB-argD-cpa-argF.
摘要
从枯草芽孢杆菌染色体DNA在表达质粒pRK9中的部分Sau3A基因文库中，分离出四个携带argA - argF - cpa簇重叠片段的杂交质粒。这些杂交质粒及其缺失衍生物在大肠杆菌精氨酸营养缺陷型中的互补模式确定了基因顺序为argC - argA - argE - argB - argD - cpa - argF。
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Studies of genetic-units controlling arginine biosynthesis in Bacillus subtilis.枯草芽孢杆菌中控制精氨酸生物合成的遗传单位的研究。
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Map locations of some mutations conferring resistance to arginine hydroxamate in Bacillus subtilis 168.枯草芽孢杆菌168中一些对精氨酸异羟肟酸盐具有抗性的突变的图谱定位。
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Bacillus subtilis 168 mutants resistant to arginine hydroxamate in the presence of ornithine or citrulline.在鸟氨酸或瓜氨酸存在的情况下，对羟胺精氨酸具有抗性的枯草芽孢杆菌168突变体。
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Plasmids containing the trp promoters of Escherichia coli and Serratia marcescens and their use in expressing cloned genes.含有大肠杆菌和粘质沙雷氏菌色氨酸启动子的质粒及其在表达克隆基因中的应用。
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The carB gene of Escherichia coli: a duplicated gene coding for the large subunit of carbamoyl-phosphate synthetase.大肠杆菌的carB基因：一个编码氨甲酰磷酸合成酶大亚基的重复基因。
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Cloning of a Bacillus subtilis restriction fragment complementing auxotrophic mutants of eight Escherichia coli genes of arginine biosynthesis.枯草芽孢杆菌一个限制片段的克隆，该片段可互补大肠杆菌精氨酸生物合成八个基因的营养缺陷型突变体。
Mol Gen Genet. 1984;197(1):82-9. doi: 10.1007/BF00327926.
10
Transcription analysis of a Bacillus subtilis arg gene following cloning in Escherichia coli in an initially unstable hybrid plasmid.在最初不稳定的杂种质粒中克隆于大肠杆菌后对枯草芽孢杆菌arg基因的转录分析。
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