Brown Shawn P, Leopold Devin R, Busby Posy E
Department of Biological Sciences, University of Memphis, Memphis, TN, USA.
Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR, USA.
Methods Mol Biol. 2018;1848:39-51. doi: 10.1007/978-1-4939-8724-5_4.
High-throughput sequencing of taxon-specific loci, or DNA metabarcoding, has become an invaluable tool for investigating the composition of plant-associated fungal communities and for elucidating plant-fungal interactions. While sequencing fungal communities has become routine, there remain numerous potential sources of systematic error that can introduce biases and compromise metabarcoding data. This chapter presents a protocol for DNA metabarcoding of the leaf mycobiome based on current best practices to minimize errors through careful laboratory practices and validation.
对特定分类群位点进行高通量测序，即DNA宏条形码分析，已成为研究植物相关真菌群落组成和阐明植物 - 真菌相互作用的一项极有价值的工具。虽然对真菌群落进行测序已成为常规操作，但仍存在许多潜在的系统误差来源，这些误差可能会引入偏差并影响宏条形码数据。本章基于当前的最佳实践方法，介绍一种用于叶片真菌群落DNA宏条形码分析的方案，通过谨慎的实验室操作和验证来尽量减少误差。
