Sakarya University, Biomedical, Magnetic and Semi Conductive Materials Research Center (BIMAS-RC), Esentepe Campus, 54187, Sakarya, Turkey.
Sakarya University, Faculty of Engineering, Department of Metallurgy and Materials Engineering, Esentepe Campus, 54187, Sakarya, Turkey.
Mater Sci Eng C Mater Biol Appl. 2018 Nov 1;92:757-768. doi: 10.1016/j.msec.2018.07.020. Epub 2018 Jul 12.
In this study, we describe new collagen/functionalized multiwalled carbon nanotube/chitosan/hydroxyapatite (Col/f-MWCNT/CS/HA) composite scaffolds which were fabricated by freezing (-40 °C at 0.9 °C/min) and lyophilization (48 h, 0 °C and 200 mtorr). The compressive stresses (from 523 to 1112 kPa), swelling (from 513.9 ± 27 to 481.05 ± 25%), porosity (from 98 ± 0.15 to 95.7 ± 0.1%), contact angle (from 87.8 to 76.7°) properties examined before and after biomineralization for comparison 3D porous Col, CS, Col/f-MWCNT and Col/f-MWCNT/CS scaffolds. Biomineralization was performed by biomimetic method in concentrated SBF (10 × SBF, at 37 °C and 6.5 pH). XRD, SEM, EDS, FTIR, TGA, Optical microscopy and BET results showed that compared to Col, CS and Col/f-MWCNT scaffolds, Col/f-MWCNT/CS scaffolds had higher in vitro bioactivity, large surface area (11.746 m/g) and a good pore volume (0.026 cc/g), interconnected porous microstructure (with 20-350 μm pore size) and incorporates the advantageous properties of both Col, f-MWCNT, CS and HA. Finally, the methyl thiazolyl tetrazolium (MTT) assay was performed to evaluate scaffolds cytotoxicity which showed that Col/f-MWCNT/CS scaffolds have the best biocompatibility.
在这项研究中，我们描述了新的胶原/功能化多壁碳纳米管/壳聚糖/羟基磷灰石（Col/f-MWCNT/CS/HA）复合支架，这些支架是通过冷冻（-40°C 时 0.9°C/min）和冻干（48 小时，0°C 和 200 mtorr）制备的。比较了 3D 多孔 Col、CS、Col/f-MWCNT 和 Col/f-MWCNT/CS 支架在生物矿化前后的压缩应力（从 523 到 1112kPa）、溶胀（从 513.9±27 到 481.05±25%）、孔隙率（从 98±0.15 到 95.7±0.1%）和接触角（从 87.8 到 76.7°）特性。生物矿化是通过在浓缩 SBF（10×SBF，37°C 和 6.5 pH）中进行仿生方法进行的。XRD、SEM、EDS、FTIR、TGA、光学显微镜和 BET 结果表明，与 Col、CS 和 Col/f-MWCNT 支架相比，Col/f-MWCNT/CS 支架具有更高的体外生物活性、更大的比表面积（11.746 m/g）和良好的孔体积（0.026 cc/g）、互连多孔微观结构（具有 20-350 μm 的孔径）和结合了 Col、f-MWCNT、CS 和 HA 的优势特性。最后，进行了甲基噻唑基四唑（MTT）测定来评估支架的细胞毒性，结果表明 Col/f-MWCNT/CS 支架具有最佳的生物相容性。
