Chongqing Key Laboratory of Oral Diseases and Biomedical Science, Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Stomatological Hospital of Chongqing Medical University, 426#, Songshi North Road, Chongqing, 401147, People's Republic of China.
Department of Stomatology, Affiliated Hospital of Chengdu University of TCM, No. 39 Shierqiao Road, Chengdu, 610075, People's Republic of China.
Ann Biomed Eng. 2017 Sep;45(9):2075-2087. doi: 10.1007/s10439-017-1866-9. Epub 2017 Jun 15.
Current bone regeneration strategies faced major challenges in fabricating the bionic scaffolds with nano-structure, constituents and mechanical features of native bone. In this study, we developed a new porous scaffold by adding the multi-walled carbon nanotube (MWCNT) into collagen (Col)/hydroxyapatite (HA) composites. Data showed that 0.5%CNT/Col/HA (0.5%CNT) group was approximately tenfolds stiffer than Col-HA, and it was superior in promoting bone marrow mesenchymal stem proliferation and spreading, mRNA and protein expressions of bone sialoprotein (BSP) and osteocalcin (OCN) than Col-HA group. Moreover, we utilized 0.5%CNT composite to repair the rat calvarial defects (8 mm diameter) in vivo, and observed the new bone formation by 3D reconstruction of micro CT, HE and Masson staining, and BSP, OCN by immunohistochemical analysis. Results showed that newly formed bone in 0.5%CNT group was significantly higher than that in Col-HA group at 12 weeks. These findings highlighted a promising strategy in healing of large area bone defect with MWCNT added into the Col-HA scaffold as they possessed the combined effects of mechanical strength and osteogenicity.
目前，骨再生策略在制造具有天然骨的纳米结构、成分和力学特性的仿生支架方面面临着重大挑战。在这项研究中，我们通过在胶原（Col）/羟基磷灰石（HA）复合材料中添加多壁碳纳米管（MWCNT）来开发一种新的多孔支架。数据显示，0.5%CNT/Col/HA（0.5%CNT）组的刚度比 Col-HA 组大约高出十倍，并且在促进骨髓间充质干细胞增殖和扩散、骨涎蛋白（BSP）和骨钙素（OCN）的 mRNA 和蛋白表达方面优于 Col-HA 组。此外，我们利用 0.5%CNT 复合材料在体内修复大鼠颅骨缺损（8mm 直径），并通过微 CT、HE 和 Masson 染色以及免疫组织化学分析 BSP、OCN 对新骨形成进行 3D 重建。结果表明，在 12 周时，0.5%CNT 组的新骨形成明显高于 Col-HA 组。这些发现强调了一种有前途的策略，即在 Col-HA 支架中添加 MWCNT 以治疗大面积骨缺损，因为它们具有机械强度和成骨作用的综合效果。
