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乙醇脱氢酶I通过对乙醛进行解毒作用，在低氧条件下促进生长和孢子形成。

Ethanol Dehydrogenase I Contributes to Growth and Sporulation Under Low Oxygen Condition via Detoxification of Acetaldehyde in .

作者信息

Zhang Erhao, Cao Yueqing, Xia Yuxian

机构信息

School of Life Sciences, Chongqing University, Chongqing, China.

Chongqing Engineering Research Center for Fungal Insecticides, Chongqing, China.

出版信息

Front Microbiol. 2018 Aug 21;9:1932. doi: 10.3389/fmicb.2018.01932. eCollection 2018.

DOI:10.3389/fmicb.2018.01932
PMID:30186258
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6110892/
Abstract

The entomopathogenic fungi encounter hypoxic conditions in both nature and artificial culture. Alcohol dehydrogenases (ADHs) are a group of oxidoreductases that occur in many organisms. Here we demonstrate that an alcohol dehydrogenase I, , in the locust-specific fungal pathogen, , functions in acetaldehyde detoxification mechanism under hypoxic conditions in growth and sporulation. The was highly expressed in sporulation stage under hypoxic conditions. Compared with a wild-type strain, the Δ mutant showed inhibited growth and sporulation under hypoxic conditions, but no impairment under normal conditions. Under hypoxic conditions, Δ mutant produced significant decreased alcohol, but significant increased acetaldehyde compared to wild type. was sensitive to exogenous acetaldehyde, exhibiting an inhibited growth and sporulation with acetaldehyde added in the medium. MaADH1 did not affect virulence. Our results indicated that the was critical to growth and sporulation under hypoxic stress by detoxification of acetaldehyde in .

摘要

昆虫病原真菌在自然环境和人工培养中都会遇到缺氧条件。酒精脱氢酶（ADHs）是一类存在于许多生物体中的氧化还原酶。在此我们证明，在蝗虫特异性真菌病原体中，一种酒精脱氢酶I（ ）在生长和孢子形成的缺氧条件下的乙醛解毒机制中发挥作用。该酶在缺氧条件下的孢子形成阶段高度表达。与野生型菌株相比，Δ突变体在缺氧条件下生长和孢子形成受到抑制，但在正常条件下无损伤。在缺氧条件下，Δ突变体产生的酒精显著减少，但与野生型相比乙醛显著增加。该酶对外源乙醛敏感，在培养基中添加乙醛时生长和孢子形成受到抑制。MaADH1不影响毒力。我们的结果表明，该酶通过解毒蝗虫体内的乙醛对缺氧胁迫下的生长和孢子形成至关重要。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f41d/6110892/65f522555499/fmicb-09-01932-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f41d/6110892/d349d219ab90/fmicb-09-01932-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f41d/6110892/27829785d998/fmicb-09-01932-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f41d/6110892/fccf94601abf/fmicb-09-01932-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f41d/6110892/d5d425506f86/fmicb-09-01932-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f41d/6110892/0c09113f90c3/fmicb-09-01932-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f41d/6110892/65f522555499/fmicb-09-01932-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f41d/6110892/d349d219ab90/fmicb-09-01932-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f41d/6110892/27829785d998/fmicb-09-01932-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f41d/6110892/fccf94601abf/fmicb-09-01932-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f41d/6110892/d5d425506f86/fmicb-09-01932-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f41d/6110892/0c09113f90c3/fmicb-09-01932-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f41d/6110892/65f522555499/fmicb-09-01932-g006.jpg

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