Amirou A, Bnouham M, Legssyer A, Ziyyat A, Aziz M, Berrabah M, Mekhfi H
Laboratory of Physiology, Genetic and Ethnopharmacology, Faculty of Sciences, Mohammed the First University, Oujda, Morocco.
Laboratory of Mineral Solid and Analytical Chemistry, Department of Chemistry, Faculty of Sciences, Mohammed the First University, Oujda, Morocco.
Evid Based Complement Alternat Med. 2018 Aug 14;2018:7313517. doi: 10.1155/2018/7313517. eCollection 2018.
Platelets have an important role in thrombosis and haemostasis. Hyperactivity of the platelets has been associated with thromboembolic diseases and represents the main cause of complications of cardiovascular diseases. Crude aqueous extract (CAE) of root bark was evaluated for bleeding time, antiaggregant activity by using agonists, thrombin, ADP, collagen, or arachidonic acid ( and ), and anticoagulant activity by measuring the clotting parameters: activated partial thromboplastin time, prothrombin time, thrombin time, and fibrinogen dosage ( and ). The result of this study reported that the strongest antiaggregant effect of CAE was observed on the ADP-induced aggregation with inhibitions up to 90 %, while, in ex vivo experiments, the inhibition (more than 80 %) was observed with all agonists. Anticoagulant effect of CAE significantly prolonged the TT and decreased the fibrinogen level and without interfering with APTT and PT. The bleeding time in mice and rats was significantly increased by CAE. The antiplatelet and anticoagulant effect observed in this study suggest that could have antithrombotic and/or thrombolytic activities and provide an alternative therapy against thrombotic complications related to cardiovascular diseases.
血小板在血栓形成和止血过程中发挥着重要作用。血小板的过度活跃与血栓栓塞性疾病相关，是心血管疾病并发症的主要原因。对根皮的粗水提取物（CAE）进行了评估，测定其出血时间、使用凝血酶、二磷酸腺苷（ADP）、胶原蛋白或花生四烯酸（及）等激动剂时的抗聚集活性，以及通过测量凝血参数（活化部分凝血活酶时间、凝血酶原时间、凝血酶时间和纤维蛋白原剂量（及））来评估其抗凝活性。本研究结果表明，CAE对ADP诱导的聚集具有最强的抗聚集作用，抑制率高达90%，而在体外实验中，对所有激动剂的抑制率均超过80%。CAE的抗凝作用显著延长了凝血酶时间，并降低了纤维蛋白原水平（及），同时不影响活化部分凝血活酶时间和凝血酶原时间。CAE显著延长了小鼠和大鼠的出血时间。本研究中观察到的抗血小板和抗凝作用表明，（此处原文可能有误，推测应为CAE）可能具有抗血栓形成和/或溶栓活性，并为治疗与心血管疾病相关的血栓并发症提供了一种替代疗法。
