Amirou Asmae, Razzok El Mahdi, Legssyer Abdelkhaleq, Ziyyat Abderrahim, Aziz Mohammed, Bnouham Mohamed, Zaid Younes, Berrabah Mohamed, Mekhfi Hassane
Laboratory of Bio-Resources, Biotechnology, Ethnopharmacology and Health, Mohammed the First University Faculty of Sciences, Oujda, Morocco.
Laboratory of Epidemiology, Clinical Research, and Public Health, Mohammed the First University, Faculty of Medicine and Pharmacy, Oujda, Morocco.
Adv Pharmacol Pharm Sci. 2023 Jan 3;2023:5644803. doi: 10.1155/2023/5644803. eCollection 2023.
Thrombosis is the formation of a clot within a blood vessel. Antithrombotic drugs are used for treating thrombosis, which can be the cause of hemorrhage. Currently, there is a need to discover novel antithrombotic drugs. Walnut is widely used to treat a wide range of health complaints. In this study, walnut bark extract was tested in hemostasis parameters: platelets adhesion, aggregation, and plasmatic coagulation in human blood. The crude aqueous extract of walnut bark was prepared by infusion and tested on hemostasis. Through blood collection from healthy volunteer donors, we studied different parameters of the primary hemostasis: platelet adhesion on the collagen-coated surface under flow, ADP, collagen, thrombin, and arachidonic acid-induced platelet aggregation, and of the secondary hemostasis by measuring prothrombin time (PT) and activated partial thromboplastin (APTT) parameters. All experiments are realized in the absence and presence of the extract and repeated at least twice. The obtained data showed that the extract (1 and 2 mg/mL) significantly ( < 0.001) reduced the activated platelet adhesion on the collagen-coated surface. In the same way, the effect of the extract on platelet aggregation seems to depend on its concentration and on the nature of the agonist. The strongest inhibition of aggregation was observed in the case of collagen at 1 mg/mL, while there was no observed effect on arachidonic acid-induced aggregation. Moreover, the extract (1 mg/mL) affects the extrinsic, intrinsic, and common pathways of the human blood coagulation cascade by extending significantly ( < 0.001), both PT and APTT times. This study provides evidence that walnut bark extract, by its antiadhesive, antiaggregant, and anticoagulant activities, could be considered as a serious source of biological compounds for the prevention and treatment of thrombosis.
血栓形成是指在血管内形成血凝块。抗血栓药物用于治疗血栓形成，而血栓形成可能是出血的原因。目前，需要发现新型抗血栓药物。核桃被广泛用于治疗多种健康问题。在本研究中，对核桃树皮提取物进行了止血参数测试：人血中的血小板黏附、聚集和血浆凝固。通过浸泡制备了核桃树皮的粗水提取物，并对其进行了止血测试。通过从健康志愿者捐献者采集血液，我们研究了初级止血的不同参数：在流动状态下血小板在胶原包被表面的黏附、二磷酸腺苷（ADP）、胶原、凝血酶和花生四烯酸诱导的血小板聚集，以及通过测量凝血酶原时间（PT）和活化部分凝血活酶时间（APTT）参数研究次级止血。所有实验均在有提取物和无提取物的情况下进行，并至少重复两次。获得的数据表明，提取物（1和2毫克/毫升）显著（<0.001）降低了活化血小板在胶原包被表面的黏附。同样，提取物对血小板聚集的影响似乎取决于其浓度和激动剂的性质。在1毫克/毫升胶原的情况下观察到最强的聚集抑制作用，而对花生四烯酸诱导的聚集未观察到影响。此外，提取物（1毫克/毫升）通过显著延长（<0.001）PT和APTT时间，影响人凝血级联反应的外源性、内源性和共同途径。本研究提供了证据，表明核桃树皮提取物因其抗黏附、抗聚集和抗凝活性，可被视为预防和治疗血栓形成的生物化合物的重要来源。
