手性锆量子点：用于冠状病毒光学检测的新型纳米晶体。
Chiral zirconium quantum dots: A new class of nanocrystals for optical detection of coronavirus.
作者信息
Ahmed Syed Rahin, Kang Seon Woo, Oh Sangjin, Lee Jaebeom, Neethirajan Suresh
机构信息
BioNano Laboratory, School of Engineering, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
Department of Cogno-Mechatronics Engineering, Pusan National University, Busan 609-735, Republic of Korea.
出版信息
Heliyon. 2018 Aug 31;4(8):e00766. doi: 10.1016/j.heliyon.2018.e00766. eCollection 2018 Aug.
A synthetic way of chiral zirconium quantum dots (Zr QDs) was presented for the first time using L(+)-ascorbic acid acts as a surface as well as chiral ligands. Different spectroscopic and microscopic analysis was performed for thorough characterization of Zr QDs. As-synthesized QDs exhibited fluorescence and circular dichroism properties, and the peaks were located at 412 nm and 352 nm, respectively. MTT assay was performed to test the cytotoxicity of the synthesized Zr QDs against rat brain glioma C6 cells. Synthesized QDs was further conjugated with anti-infectious bronchitis virus (IBV) antibodies of coronavirus to form an immunolink at the presence of the target analyte and anti-IBV antibody-conjugated magneto-plasmonic nanoparticles (MPNPs). The fluorescence properties of immuno-conjugated QD-MP NPs nanohybrids through separation by an external magnetic field enabled biosensing of coronavirus with a limit of detection of 79.15 EID/50 μL.
首次提出了一种合成手性锆量子点（Zr QDs）的方法，该方法使用L(+)-抗坏血酸作为表面以及手性配体。对Zr QDs进行了不同的光谱和显微镜分析以进行全面表征。合成的量子点表现出荧光和圆二色性特性，其峰分别位于412 nm和352 nm处。进行MTT试验以测试合成的Zr QDs对大鼠脑胶质瘤C6细胞的细胞毒性。合成的量子点在存在目标分析物和抗传染性支气管炎病毒（IBV）抗体偶联的磁等离子体纳米颗粒（MPNPs）的情况下，进一步与冠状病毒的抗IBV抗体偶联以形成免疫连接。通过外部磁场分离的免疫偶联QD-MP NPs纳米杂化物的荧光特性能够实现冠状病毒的生物传感，检测限为79.15 EID/50 μL。
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