Corrocher R, Casaril M, Bellisola G, Gabrielli G B, Nicoli N, Guidi G C, De Sandre G
Cancer. 1986 Oct 15;58(8):1658-62. doi: 10.1002/1097-0142(19861015)58:8<1658::aid-cncr2820580814>3.0.co;2-7.
Catalase (CAT), glutathione-peroxidase (GSH-Px) activity and reduced glutathione content (GSH) were measured in patients who had hepatocellular carcinoma, and values compared with those of normal liver and liver adjacent to neoplastic tissue. The results showed a remarkable reduction of CAT in tumor and corresponding tumor-free tissue (P less than 0.001 and P less than 0.02, respectively). All neoplastic samples had a significant lower activity of CAT than the corresponding adjacent tumor-free tissue (P less than 0.05). The GSH-Px activity of tumor tissue also was lower than normal (P less than 0.001) but similar to that of adjacent tissue. No correlation was noted between the two enzyme activities. Glutathione content was extremely low in tumor (P less than 0.001) and even in tumor-free tissue (P less than 0.05) when compared with normal liver. In all cases the content of GSH in neoplastic tissue was lower than that of the corresponding tumor-free tissue (P less than 0.05). Whereas in normal liver the activity of GSH-Px was positively correlated with the content of GSH, in the neoplastic tissue such a relationship disappeared. All these findings suggest that the antioxidant system of hepatocellular carcinoma cell is severely impaired.
在肝细胞癌患者中检测了过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）活性以及还原型谷胱甘肽含量（GSH），并将这些值与正常肝脏以及肿瘤组织相邻肝脏的值进行比较。结果显示，肿瘤组织及相应的无肿瘤组织中CAT显著降低（分别为P＜0.001和P＜0.02）。所有肿瘤样本的CAT活性均显著低于相应的相邻无肿瘤组织（P＜0.05）。肿瘤组织的GSH-Px活性也低于正常组织（P＜0.001），但与相邻组织相似。两种酶活性之间未发现相关性。与正常肝脏相比，肿瘤组织中的谷胱甘肽含量极低（P＜0.001），甚至无肿瘤组织中的谷胱甘肽含量也较低（P＜0.05）。在所有病例中，肿瘤组织中的GSH含量均低于相应的无肿瘤组织（P＜0.05）。在正常肝脏中，GSH-Px活性与GSH含量呈正相关，而在肿瘤组织中这种关系消失。所有这些发现表明，肝细胞癌细胞的抗氧化系统严重受损。
