Division of Infectious Diseases, Faculty of Medicine, The University of British Columbia, Vancouver, BC V6H 3Z6, Canada.
Dipartimento di Scienze Biomediche, Università di Sassari, Viale San Pietro 43 b, 07100 Sassari, Italy.
J Neuroimmunol. 2018 Oct 15;323:49-52. doi: 10.1016/j.jneuroim.2018.07.007. Epub 2018 Jul 21.
Mycobacterium avium ssp. paratuberculosis (Map) is the etiological agent of Paratuberculosis in ruminants. Protein tyrosine phosphatase A (PtpA) and protein kinase G (PknG) are secreted proteins necessary for the survival of the pathogen within macrophages. In this study we analyzed if Map was able to grow within astrocytes and investigated on the presence of antibodies against PtpA and PknG proteins in MS and NMOSD patients by ELISA. Map was unable to proliferate in astrocytes after of 72 h post-infection, but we observed a high level of antibodies against both virulence factors, suggesting that these patients have been exposed/infected with Map.
分支杆菌 avium ssp. paratuberculosis（Map）是反刍动物副结核病的病原体。蛋白酪氨酸磷酸酶 A（PtpA）和蛋白激酶 G（PknG）是该病原体在巨噬细胞内生存所必需的分泌蛋白。在这项研究中，我们分析了 Map 是否能够在星形胶质细胞内生长，并通过 ELISA 检测了 MS 和 NMOSD 患者中针对 PtpA 和 PknG 蛋白的抗体存在情况。Map 在感染后 72 小时内无法在星形胶质细胞内增殖，但我们观察到针对这两种毒力因子的抗体水平很高，表明这些患者曾接触/感染过 Map。
