Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow 226014, Uttar Pradesh, India.
King George Medical University, Lucknow 226003, Uttar Pradesh, India.
J Neuroimmunol. 2018 Oct 15;323:56-61. doi: 10.1016/j.jneuroim.2018.07.014. Epub 2018 Jul 26.
Japanese encephalitis virus (JEV) is a neurotropic mosquito-borne Flavivirus, mainly prevalent in Asia. It is the most important causative agent of acute viral encephalitis in humans. Recently, micro RNAs are discovered as a key regulator of inflammatory and immune responses in various diseases including neurological and viral infections. Thus, this study was proposed to check whether changes in cellular miRNA expression due to JE virus infection, can be detected in circulation which would be helpful in diagnosis and treatment.
miRNAs (miR-29b and miR-146a) were analyzed in the serum of JEV infected patients using quantitative reverse transcription polymerase chain reaction (RT-qPCR).
miR-146a was found significantly decreased (p = 0.0008) in JEV infected patients as compared to healthy controls whereas miR-29b was significantly increased (p = 0.001) in JEV patients recovered with neurological sequelae when compared to those recovered without sequelae.
In conclusion, miRNA can be measured in serum. Studying microRNAs will provide novel information and help us to identify the components that can serve as biomarkers and can lead to new discovery in controlling disease recovery.
日本脑炎病毒（JEV）是一种嗜神经的蚊媒黄病毒，主要流行于亚洲。它是人类急性病毒性脑炎的最重要病原体。最近，microRNAs 被发现是包括神经和病毒感染在内的各种疾病中炎症和免疫反应的关键调节因子。因此，本研究旨在检查由于 JEV 感染而导致的细胞 microRNA 表达的变化是否可以在循环中检测到，这将有助于诊断和治疗。
使用定量逆转录聚合酶链反应（RT-qPCR）分析 JEV 感染患者血清中的 microRNAs（miR-29b 和 miR-146a）。
与健康对照组相比，JEV 感染患者的 miR-146a 显著降低（p=0.0008），而有神经后遗症的 JEV 患者恢复后 miR-29b 显著升高（p=0.001），与无后遗症的患者相比。
总之，miRNA 可以在血清中测量。研究 microRNAs 将提供新的信息，并帮助我们确定可以作为生物标志物的成分，并为控制疾病恢复带来新的发现。
