Strnad C F, Parente J E, Wong K
FEBS Lett. 1986 Sep 29;206(1):20-4. doi: 10.1016/0014-5793(86)81332-1.
Fluoride activation of neutrophils was found to be associated with phosphoinositide turnover, as monitored by the time-dependent accumulation of inositol phosphates. Unlike phosphoinositide turnover induced by the chemotactic peptide, formylmethionylleucylphenylalanine, that induced by fluoride was not inhibited by pretreatment with pertussis toxin. The translocation of protein kinase C activity from the cytosolic to the membrane compartment was also observed in fluoride-stimulated cells. We have proposed that the mode of action of this halide ion involves interaction with a GTP-binding protein which serves as an intermediary unit between the receptors for inflammatory stimuli and the phosphoinositide-specific phosphodiesterase.
研究发现，氟化物对中性粒细胞的激活与磷酸肌醇转换有关，这可通过肌醇磷酸随时间的积累来监测。与趋化肽甲酰甲硫氨酰亮氨酰苯丙氨酸诱导的磷酸肌醇转换不同，氟化物诱导的磷酸肌醇转换不受百日咳毒素预处理的抑制。在氟化物刺激的细胞中还观察到蛋白激酶C活性从胞质向膜区室的转位。我们提出，这种卤离子的作用方式涉及与一种GTP结合蛋白相互作用，该蛋白作为炎症刺激受体和磷酸肌醇特异性磷酸二酯酶之间的中间单元。
