喉癌中葡萄糖转运蛋白1、缺氧诱导因子1α和T-box转录因子21基因多态性的研究
Investigation of GLUT1, HIF1α and TBX21 Gene Polymorphisms in Laryngeal Cancer.
作者信息
Uslu Cihan, Tüz Mustafa, Yasan Hasan, Okur Erdoğan
机构信息
Department of Otorhinolaryngology-Head and Neck Surgery, Süleyman Demirel University School of Medicine, Isparta, Turkey.
出版信息
Turk Arch Otorhinolaryngol. 2018 Jun;56(2):70-74. doi: 10.5152/tao.2018.3177. Epub 2018 Jun 1.
OBJECTIVE
We aimed to investigate the association of the frequency of three single-nucleotide polymorphisms [glucose transporter isoform 1 (GLUT1) reference single-nucleotide polymorphism (rs) 710218, hypoxia-inducible factor 1 alpha (HIF1α) rs11549465, and T-box transcription factor protein 21 (TBX21) rs17250932], which have been proved to be related with various benign and malignant diseases, with the development of laryngeal cancer and its size and grade.
METHODS
In this study, we included 35 patients with laryngeal cancer and 35 volunteers at least 30 years old who had smoked for at least 20 years. DNA was obtained from the blood samples of the participants using an isolation kit. Then, polymorphisms for both the groups were determined using real-time polymerase chain reaction.
RESULTS
No significant differences were detected regarding the genotype and allele frequencies in the three polymorphisms assessed between the two groups. In the patient group, on examining the association of polymorphisms with tumor size and grade, no significant relation was observed in three polymorphisms regarding the related parameters.
CONCLUSION
GLUT1, HIF1α, and TBX21 polymorphisms have no impact on the development of laryngeal cancer.
目的
我们旨在研究三种单核苷酸多态性[葡萄糖转运蛋白1型（GLUT1）参考单核苷酸多态性（rs）710218、缺氧诱导因子1α（HIF1α）rs11549465和T盒转录因子蛋白21（TBX21）rs17250932]的频率与喉癌发生、大小及分级之间的关联，这些多态性已被证明与多种良性和恶性疾病相关。
方法
在本研究中，我们纳入了35例喉癌患者以及35名年龄至少30岁、吸烟至少20年的志愿者。使用分离试剂盒从参与者的血液样本中提取DNA。然后，通过实时聚合酶链反应确定两组的多态性。
结果
在评估的三种多态性中，两组之间的基因型和等位基因频率未检测到显著差异。在患者组中，检查多态性与肿瘤大小和分级的关联时，在三种多态性与相关参数之间未观察到显著关系。
结论
GLUT1、HIF1α和TBX21多态性对喉癌的发生没有影响。
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