噬菌体Mu的mom基因：甲基化依赖性表达的机制。
The mom gene of bacteriophage Mu: the mechanism of methylation-dependent expression.
作者信息
Seiler A, Blöcker H, Frank R, Kahmann R
出版信息
EMBO J. 1986 Oct;5(10):2719-28. doi: 10.1002/j.1460-2075.1986.tb04556.x.
Abstract
Transcription of the DNA modification gene (mom) of bacteriophage Mu requires methylation of three GATC sites upstream of the mom promoter by the Escherichia coli deoxyadenosine methylation function (Dam). The three sites map within a 40-bp segment termed region I. Small deletions, inversions, duplications and specific point mutations have been introduced in region I. Their effect on mom expression has been studied in dam+ and dam strains. Dam-dependent expression of the mom gene requires a specific arrangement of the three GATC sites and the presence of the methylated base in at least two of the three sites. We show that mom specific modification is regulated by a host protein. The Mom function is expressed in dam strains if they are defective in one component of the methylation-instructed mismatch correction system, mutH. We suggest that the product of mutH functions as a transcriptional repressor by binding to region I.
摘要
噬菌体Mu的DNA修饰基因（mom）的转录需要大肠杆菌脱氧腺苷甲基化功能（Dam）对mom启动子上游的三个GATC位点进行甲基化。这三个位点位于一个称为区域I的40碱基对片段内。已在区域I中引入了小缺失、倒位、重复和特定点突变。在dam+和dam菌株中研究了它们对mom表达的影响。mom基因的Dam依赖性表达需要三个GATC位点的特定排列以及三个位点中至少两个位点存在甲基化碱基。我们表明，mom特异性修饰受宿主蛋白调控。如果dam菌株在甲基化指导的错配修复系统的一个组分mutH中存在缺陷，则Mom功能会表达。我们认为mutH的产物通过与区域I结合而作为转录阻遏物发挥作用。
相似文献
1
The mom gene of bacteriophage Mu: the mechanism of methylation-dependent expression.噬菌体Mu的mom基因：甲基化依赖性表达的机制。
EMBO J. 1986 Oct;5(10):2719-28. doi: 10.1002/j.1460-2075.1986.tb04556.x.
2
Methylation dependent expression of the mom gene of bacteriophage Mu: deletions downstream from the methylation sites affect expression.噬菌体Mu的mom基因的甲基化依赖性表达：甲基化位点下游的缺失影响表达。
Nucleic Acids Res. 1984 Apr 25;12(8):3535-50. doi: 10.1093/nar/12.8.3535.
3
S1 nuclease mapping of the phage Mu mom gene promoter: a model for the regulation of mom expression.噬菌体Mu mom基因启动子的S1核酸酶图谱分析：mom表达调控模型
Gene. 1984 Jul-Aug;29(1-2):185-98. doi: 10.1016/0378-1119(84)90179-3.
4
The sequence and mom-transactivation function of the C gene of bacteriophage Mu.噬菌体Mu C基因的序列及非转激活功能
Gene. 1986;43(1-2):59-67. doi: 10.1016/0378-1119(86)90008-9.
5
Transcription initiation of Mu mom depends on methylation of the promoter region and a phage-coded transactivator.Mu噬菌体妈妈基因的转录起始依赖于启动子区域的甲基化作用以及一种噬菌体编码的反式激活因子。
Nature. 1983 Jan 27;301(5898):344-7. doi: 10.1038/301344a0.
6
The Escherichia coli MutH protein is not the repressor of the bacteriophage Mu mom operon.大肠杆菌MutH蛋白不是噬菌体Mu mom操纵子的阻遏物。
Gene. 1989 Jul 15;79(2):381-3. doi: 10.1016/0378-1119(89)90221-7.
7
Analysis of the methylation-regulated Mu mom transcript.甲基化调控的Mu mom转录本分析。
Cell. 1984 Jan;36(1):189-96. doi: 10.1016/0092-8674(84)90088-6.
8
The Escherichia coli regulatory protein OxyR discriminates between methylated and unmethylated states of the phage Mu mom promoter.大肠杆菌调节蛋白OxyR能够区分噬菌体Mu mom启动子的甲基化和未甲基化状态。
EMBO J. 1989 Aug;8(8):2403-10. doi: 10.1002/j.1460-2075.1989.tb08370.x.
9
Bacteriophage Mu-induced modification of DNA is dependent upon a host function.噬菌体Mu诱导的DNA修饰依赖于宿主功能。
J Bacteriol. 1978 Oct;136(1):423-8. doi: 10.1128/jb.136.1.423-428.1978.
10
Counterselection of GATC sequences in enterobacteriophages by the components of the methyl-directed mismatch repair system.甲基导向错配修复系统的组分对肠道噬菌体中GATC序列的反选择作用
J Mol Evol. 1991 Aug;33(2):125-32. doi: 10.1007/BF02193626.
引用本文的文献
1
Escherichia coli OxyR modulation of bacteriophage Mu mom expression in dam+ cells can be attributed to its ability to bind hemimethylated Pmom promoter DNA.大肠杆菌OxyR对dam+细胞中噬菌体Mu mom表达的调控可归因于其结合半甲基化Pmom启动子DNA的能力。
Nucleic Acids Res. 1997 Nov 1;25(21):4385-8. doi: 10.1093/nar/25.21.4385.
2
Mutation frequency decline in Escherichia coli. I. Effects of defects in mismatch repair.大肠杆菌中的突变频率下降。I.错配修复缺陷的影响。
Mol Gen Genet. 1995 Dec 20;249(6):585-90. doi: 10.1007/BF00418027.
3
In vitro transcriptional activation of the phage Mu mom promoter by C protein.C蛋白对噬菌体Mu mom启动子的体外转录激活作用。
J Bacteriol. 1994 May;176(10):2885-91. doi: 10.1128/jb.176.10.2885-2891.1994.
4
Membrane topology and assembly of the outer membrane protein OmpA of Escherichia coli K12.大肠杆菌K12外膜蛋白OmpA的膜拓扑结构与组装
Mol Gen Genet. 1994 Apr;243(2):127-35. doi: 10.1007/BF00280309.
5
Mutation spectrum in Escherichia coli DNA mismatch repair deficient (mutH) strain.大肠杆菌DNA错配修复缺陷（mutH）菌株中的突变谱
Nucleic Acids Res. 1987 Oct 26;15(20):8205-15. doi: 10.1093/nar/15.20.8205.
6
Nucleotide sequence of the Escherichia coli mutH gene.大肠杆菌mutH基因的核苷酸序列。
Nucleic Acids Res. 1987 Apr 10;15(7):3073-84. doi: 10.1093/nar/15.7.3073.
7
Transcripts within the replication origin, oriC, of Escherichia coli.大肠杆菌复制起点oriC内的转录本。
Nucleic Acids Res. 1987 Mar 25;15(6):2479-97. doi: 10.1093/nar/15.6.2479.
8
The Escherichia coli regulatory protein OxyR discriminates between methylated and unmethylated states of the phage Mu mom promoter.大肠杆菌调节蛋白OxyR能够区分噬菌体Mu mom启动子的甲基化和未甲基化状态。
EMBO J. 1989 Aug;8(8):2403-10. doi: 10.1002/j.1460-2075.1989.tb08370.x.
9
dam methylation and the initiation of DNA replication on oriC plasmids.dam甲基化与oriC质粒上DNA复制的起始
Mol Gen Genet. 1989 Apr;216(2-3):217-23. doi: 10.1007/BF00334359.
10
Role of bacteriophage Mu C protein in activation of the mom gene promoter.噬菌体Mu C蛋白在mom基因启动子激活中的作用。
J Bacteriol. 1989 Apr;171(4):2019-27. doi: 10.1128/jb.171.4.2019-2027.1989.
本文引用的文献
1
Segregation of Lambda Lysogenicity during Bacterial Recombination in Escherichia Coli K12.大肠杆菌K12中细菌重组过程中λ原噬菌体溶源性的分离
Genetics. 1954 Jul;39(4):429-39. doi: 10.1093/genetics/39.4.429.
2
High-frequency generalised transduction by bacteriophage T4.噬菌体T4介导的高频广义转导
Nature. 1979 Jul 5;280(5717):80-2. doi: 10.1038/280080a0.
3
Replication origin of the Escherichia coli K-12 chromosome: the size and structure of the minimum DNA segment carrying the information for autonomous replication.大肠杆菌K-12染色体的复制起点：携带自主复制信息的最小DNA片段的大小和结构
Mol Gen Genet. 1980 Apr;178(1):9-20. doi: 10.1007/BF00267207.
4
Escherichia coli mutator mutants deficient in methylation-instructed DNA mismatch correction.缺乏甲基化指导的DNA错配修复的大肠杆菌突变体
Proc Natl Acad Sci U S A. 1980 Feb;77(2):1063-7. doi: 10.1073/pnas.77.2.1063.
5
DNA methyltransferase-dependent transcription of the phage Mu mom gene.噬菌体Mu mom基因的DNA甲基转移酶依赖性转录
Proc Natl Acad Sci U S A. 1982 Sep;79(18):5518-21. doi: 10.1073/pnas.79.18.5518.
6
Changing the DNA-binding specificity of a repressor.改变阻遏物的DNA结合特异性。
Cell. 1983 Dec;35(3 Pt 2):777-83. doi: 10.1016/0092-8674(83)90110-1.
7
Mapping of the modification function of temperate phage Mu-1.温和噬菌体Mu-1修饰功能的图谱绘制
Mol Gen Genet. 1980 Jan;177(2):351-3. doi: 10.1007/BF00267450.
8
How the lambda repressor and cro work.λ阻遏蛋白和Cro蛋白是如何发挥作用的。
Cell. 1980 Jan;19(1):1-11. doi: 10.1016/0092-8674(80)90383-9.
9
Different base/base mismatches are corrected with different efficiencies by the methyl-directed DNA mismatch-repair system of E. coli.大肠杆菌的甲基化导向DNA错配修复系统对不同的碱基/碱基错配进行校正的效率不同。
Cell. 1984 Oct;38(3):879-87. doi: 10.1016/0092-8674(84)90283-6.
10
Purification and characterization of the unusual deoxynucleoside, alpha-N-(9-beta-D-2'-deoxyribofuranosylpurin-6-yl)glycinamide, specified by the phage Mu modification function.由噬菌体Mu修饰功能所指定的异常脱氧核苷α-N-(9-β-D-2'-脱氧呋喃核糖基嘌呤-6-基)甘氨酰胺的纯化与特性鉴定
Proc Natl Acad Sci U S A. 1983 Dec;80(24):7400-4. doi: 10.1073/pnas.80.24.7400.
Zotero 插件