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碳源、C/N 比、硝酸盐、温度和 pH 值对异养反硝化过程中 NO 排放和功能反硝化基因的影响。

Effects of carbon source, C/N ratio, nitrate, temperature, and pH on NO emission and functional denitrifying genes during heterotrophic denitrification.

机构信息

a Department of Environmental Science and Engineering , Ewha Womans University , Seoul , Republic of Korea.

出版信息

J Environ Sci Health A Tox Hazard Subst Environ Eng. 2019;54(1):16-29. doi: 10.1080/10934529.2018.1503903. Epub 2018 Sep 10.

DOI:10.1080/10934529.2018.1503903
PMID:30199323
Abstract

The effects of operational parameters such as carbon source, C/N ratio, initial nitrate concentration, temperature, and pH value on heterotrophic denitrification and functional denitrifying genes were evaluated. When methanol was used as the sole carbon source, complete denitrification was performed in a short time without nitrous oxide (NO) emission. Complete denitrification was performed at high C/N ratios (5.14 and 12.85) and low initial nitrate concentrations (75.9 and 151.6 mg N L). The denitrification rate was not temperature-sensitive in the range of 25-35 °C, but tended to decrease at a low pH of 5-6. The relationships between NO emission and functional genes under various operational conditions were investigated by Pearson correlation and association network analyses. The C/N ratio was a key factor for NO emission during the heterotrophic denitrification process. This information on the denitrification performance and its association with functional gene dynamics under various operational conditions is useful for NO mitigation strategies for wastewater treatment processes.

摘要

评估了操作参数(如碳源、C/N 比、初始硝酸盐浓度、温度和 pH 值)对异养反硝化和功能反硝化基因的影响。当甲醇用作唯一碳源时,可在短时间内完成完全反硝化,且无一氧化二氮(NO)排放。在高 C/N 比(5.14 和 12.85)和低初始硝酸盐浓度(75.9 和 151.6mgN/L)下,可实现完全反硝化。在 25-35°C 的范围内,反硝化速率对温度不敏感,但在 pH 值为 5-6 时,反硝化速率往往会降低。通过 Pearson 相关性和关联网络分析研究了不同操作条件下 NO 排放与功能基因之间的关系。C/N 比是异养反硝化过程中 NO 排放的关键因素。这些关于反硝化性能及其与各种操作条件下功能基因动态关系的信息,对于废水处理过程中的 NO 减排策略很有用。

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