一种用于同时检测牛奶中黄曲霉毒素 M1 和氯霉素的灵敏双分析物免疫层析条。
A Sensitive Two-Analyte Immunochromatographic Strip for Simultaneously Detecting Aflatoxin M1 and Chloramphenicol in Milk.
机构信息
Graduate institute of Medicine, Chung Shan Medical University, Taichung 40201, Taiwan.
Graduate Institute of Toxicology, College of Medicine, National Taiwan University, Taipei 10051, Taiwan.
出版信息
Toxins (Basel). 2020 Oct 2;12(10):637. doi: 10.3390/toxins12100637.
A two-analyte immunochromatographic strip (immunostrip) was developed for the simultaneous detection of aflatoxin M1 (AFM1) and chloramphenicol (CAP) in milk. Protein conjugates (AFM1-ovalbumin (OVA) and CAP-OVA) and goat anti-rabbit IgG were respectively drawn on nitrocellulose membrane as two test lines (T and T) and a control line (C). The immunostrip was dipped into a well that contained a 200 μL milk sample, 5 μL AFM1 antibody-gold conjugates, and 8 μL CAP antibody-gold conjugates; the whole assay was completed in 15 min and the results could be interpreted visually or using a reader. This immunostrip has cut-off levels of 0.1 ng/mL and 0.5 ng/mL for AFM1 and CAP, respectively. Analysis of CAP and AFM1 in milk samples revealed that data from the immunostrip test agreed closely with those obtained from ELISA. The two-analyte immunostrip is a rapid way for on-site simultaneous detection of AFM1 and CAP in milk.
一种双分析物免疫层析条（免疫条）被开发用于同时检测牛奶中的黄曲霉毒素 M1（AFM1）和氯霉素（CAP）。蛋白质结合物（AFM1-卵清蛋白（OVA）和 CAP-OVA）和兔抗山羊 IgG 分别被绘制在硝酸纤维素膜上作为两个测试线（T 和 T）和一个控制线（C）。免疫条被浸入一个含有 200 μL 牛奶样品、5 μL AFM1 抗体-金结合物和 8 μL CAP 抗体-金结合物的孔中；整个测定在 15 分钟内完成，结果可以通过肉眼或使用读数器进行解释。该免疫条对 AFM1 和 CAP 的截断值分别为 0.1ng/mL 和 0.5ng/mL。对牛奶样品中 CAP 和 AFM1 的分析表明，免疫条试验的结果与 ELISA 获得的结果非常吻合。双分析物免疫条是一种快速的现场同时检测牛奶中 AFM1 和 CAP 的方法。
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