Department of Occupational and Environmental Medicine, Epidemiology and Hygiene, INAIL-National Institute for Insurance against Accidents at Work, 00078 Monteporzio Catone, Italy.
Int J Environ Res Public Health. 2018 Sep 6;15(9):1950. doi: 10.3390/ijerph15091950.
Phthalates are widely used in the industrial manufacture of many products. Some phthalates have shown reproductive toxicity in humans, acting as endocrine disruptors, so they were included in the authorization process defined in Reg. CE 1907/2006 (REACH). Two groups of population were recruited, before and after the inclusion of some phthalates in the authorization list in REACH: the first group of 157 volunteers was studied in 2011 and the second, 171 volunteers, in 2016. Each subject completed a questionnaire about personal lifestyle, working activities and use of chemical products. The main urinary metabolites of five phthalates were analyzed by HPLC/MS/MS: mono(2-ethylhexyl)phthalate (MEHP) and mono(2-ethyl-5-hydroxyhexyl) phthalate (MEHHP) for di(2-ethylhexyl)phthalate (DEHP) exposure; monoethylphthalate (MEP) for diethylphtahate (DEP); monobenzylphthalate (MBzP) for butylbenzylphtahalate (BBP) and dibenzylphthalate (DBzP), mono--butylphthalate (MnBP) for butylbenzylphtahalate (BBP) and di--butylphthalate (DnBP). The results show a significant difference for all metabolites between the two periods, with the exception of MEP in women. The comparison of the two sets of results shows a decrease in urinary metabolites excretion from 2011 to 2016, statistically significant for the three phthalates included in Annex XIV of REACH. DEP, not currently included in the list for authorization, maintains a constant presence in the daily life of the population, particularly for women.
邻苯二甲酸酯广泛应用于许多产品的工业制造中。一些邻苯二甲酸酯已被证明对人类具有生殖毒性，作为内分泌干扰物，因此它们被纳入了法规 (EC) 1907/2006 (REACH) 定义的授权程序中。在 REACH 授权清单中纳入某些邻苯二甲酸酯之前和之后，招募了两组人群：第一组 157 名志愿者于 2011 年进行了研究，第二组 171 名志愿者于 2016 年进行了研究。每位受试者都完成了一份关于个人生活方式、工作活动和使用化学产品的问卷。通过 HPLC/MS/MS 分析了五种邻苯二甲酸酯的主要尿代谢物：单（2-乙基己基）邻苯二甲酸酯 (MEHP) 和单（2-乙基-5-羟基己基）邻苯二甲酸酯 (MEHHP) 用于二（2-乙基己基）邻苯二甲酸酯 (DEHP) 的暴露；单乙基邻苯二甲酸酯 (MEP) 用于邻苯二甲酸二乙酯 (DEP)；单苄基邻苯二甲酸酯 (MBzP) 用于邻苯二甲酸丁基苄酯 (BBP) 和邻苯二甲酸二丁酯 (DBzP)，单丁基邻苯二甲酸酯 (MnBP) 用于邻苯二甲酸丁基苄酯 (BBP) 和邻苯二甲酸二丁酯 (DnBP)。结果表明，除了女性中的 MEP 外，两个时期的所有代谢物之间均存在显著差异。两组结果的比较表明，从 2011 年到 2016 年，尿液代谢物的排泄量减少，这在 REACH 附件 XIV 中列出的三种邻苯二甲酸酯中具有统计学意义。目前未列入授权清单的邻苯二甲酸二乙酯 (DEP) 在人群的日常生活中仍然存在，特别是在女性中。
