Institute of Biology and Medicine, College of Life and Health Sciences, Wuhan University of Science and Technology, Hubei 430081, PR China.
Institute of Biology and Medicine, College of Life and Health Sciences, Wuhan University of Science and Technology, Hubei 430081, PR China.
Gene. 2018 Dec 30;679:232-240. doi: 10.1016/j.gene.2018.09.011. Epub 2018 Sep 7.
Lung cancer remains one of the most aggressive human malignancies with a low survival rate. Hyperoside (quercetin-3-O-β-d-galactopyranoside) is a flavonol glycoside with an anti-cancer activity. The microRNA-let-7 was widely regarded as a tumor suppressor in human tumors. Here, we investigated the role of hyperoside and let-7a-5p on the lung cancer cell proliferation, cell cycle and apoptosis in A549 cells in vitro. Our results showed that hyperoside could inhibit the proliferation of A549 cells through inducing apoptosis and G1/S phase arrest. Let-7a-5p could inhibit the proliferation of A549 cells via inhibiting the process of G1/S phase. Additionally, hyperoside and let-7a-5p had a synergetic effect on suppressing the proliferation of A549 cells; microRNA-let-7a-5p directly regulated the expression of CCND1 in A549 cells. Our study illustrated that hyperoside and microRNA-let7a-5p might provide a synergistic effect on anti-cancer, which may provide a new idea for lung cancer treatment.
肺癌仍然是人类最具侵袭性的恶性肿瘤之一，其生存率较低。山柰酚-3-O-Β-D-吡喃半乳糖苷（hyperoside，quercetin-3-O-β-d-galactopyranoside）是一种具有抗癌活性的类黄酮糖苷。microRNA-let-7 被广泛认为是人类肿瘤中的肿瘤抑制因子。在这里，我们研究了山柰酚和 let-7a-5p 对体外 A549 细胞增殖、细胞周期和细胞凋亡的作用。我们的结果表明，山柰酚通过诱导细胞凋亡和 G1/S 期阻滞来抑制 A549 细胞的增殖。let-7a-5p 通过抑制 G1/S 期进程来抑制 A549 细胞的增殖。此外，山柰酚和 let-7a-5p 对抑制 A549 细胞增殖具有协同作用；microRNA-let-7a-5p 直接调控 A549 细胞中 CCND1 的表达。我们的研究表明，山柰酚和 microRNA-let7a-5p 可能对抗癌具有协同作用，这可能为肺癌治疗提供新的思路。
