Institute of Cellular and Molecular Biology, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu 221116, China.
Institute of Cellular and Molecular Biology, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu 221116, China.
Int J Biol Macromol. 2019 Jan;121:1308-1313. doi: 10.1016/j.ijbiomac.2018.09.039. Epub 2018 Sep 7.
Skeletal muscle development and growth regulatory mechanism is the focus of both animal genetics and medicine. The recent studies indicate that covalently closed circular RNAs (circRNAs) also play important role on muscle development through sequestering specific miRNAs. The present study was conducted to determine the functional roles of circZfp609, a recently identified circRNA, in the regulation of myogenesis in mouse myoblast cell line (C2C12). circZfp609 is predicted to has binding sites of miR-194-5p. circZfp609 knockdown increased the expression of Myf5 and MyoG, which indicated that circZfp609 suppressed myogenic differentiation. Via a luciferase screening assay, circZfp609 is observed to sponge to miR-194-5p with four potential binding sites. Specifically, we show that circZfp609 can sponge miR-194-5p to sequester its inhibition on BCLAF1 so as to repress the myogenic differentiation. Modulation of circZfp609 expression in muscle tissue may emerge as a potential target in breeding strategies attempting to control muscle development.
骨骼肌的发育和生长调控机制是动物遗传学和医学研究的重点。最近的研究表明，共价闭合环状 RNA（circRNA）也通过结合特定的 miRNA 在肌肉发育中发挥重要作用。本研究旨在确定circZfp609 在调控小鼠成肌细胞系（C2C12）中的肌发生中的功能作用。circZfp609 被预测具有 miR-194-5p 的结合位点。circZfp609 的敲低增加了 Myf5 和 MyoG 的表达，这表明 circZfp609 抑制了成肌分化。通过荧光素酶筛选实验，观察到 circZfp609 与 miR-194-5p 结合有四个潜在的结合位点。具体来说，我们表明 circZfp609 可以与 miR-194-5p 结合，从而抑制其对 BCLAF1 的抑制作用，从而抑制成肌分化。调节肌肉组织中的 circZfp609 表达可能成为控制肌肉发育的育种策略中的一个潜在目标。
