College of Animal Science and Technology, Shandong Agricultural University, 61 Daizong Road, Tai'an 271018, Shandong Province, China.
Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, 61 Daizong Road, Tai'an 271018, Shandong Province, China.
Viruses. 2018 Sep 10;10(9):485. doi: 10.3390/v10090485.
Tembusu virus (TMUV) is a contagious pathogen from fowl that mainly infects ducks and geese, causing symptoms of high fever, loss of appetite, retarded growth, neurological symptoms, severe duck-drop syndrome, and even death. During an epidemiological investigation of TMUV in Northern China, we isolated 11 TMUV strains from ducks, chickens, geese, sparrows, and mosquitoes (2011⁻2017). Phylogenetic analysis of the open-reading frames of genes revealed that these strains clustered into Chinese strains II. The nucleotide and amino acid homologies of of the strains ranged between 85.8⁻99.8% and 92.5⁻99.68%, respectively, which were lower than those of (86.7⁻99.9% and 96.5⁻99.9%, respectively), (87.6⁻99.9% and 98.2⁻99.8%, respectively), and (86.5⁻99.9% and 97.8⁻99.9%, respectively). Predictions of the tertiary structure of the viral proteins indicated that NS1 in 4 of 11 strains had a protein structure mutation at TAV that changed a random crimp into an alpha helix. The protein of 6 of 11 strains had a glycosylation site mutation from NTTD to NITD. Furthermore, epidemiological data suggested that TMUV has been circulating in half of China's provinces (17 of 34). Our findings, for the first time, have identified the NS1 protein as a potential hypervariable region for genetic evolution. Additionally, the territorial scope of the virus has expanded, requiring strict bio-security measures or a multivalent vaccine to control its spread.
腾格里病毒（TMUV）是一种传染性禽类病原体，主要感染鸭和鹅，引起高热、食欲不振、生长迟缓、神经症状、严重的鸭下降综合征，甚至死亡。在中国北方进行 TMUV 的流行病学调查时，我们从鸭、鸡、鹅、麻雀和蚊子中分离出 11 株 TMUV 株（2011-2017 年）。基因开放阅读框的系统发育分析表明，这些分离株聚集在中国株 II 中。株间核苷酸和氨基酸同源性分别为 85.8%-99.8%和 92.5%-99.68%，低于 （86.7%-99.9%和 96.5%-99.9%）、 （87.6%-99.9%和 98.2%-99.8%）和 （86.5%-99.9%和 97.8%-99.9%）。病毒蛋白三级结构预测表明，11 株中的 4 株 NS1 在 TAV 处发生了蛋白质结构突变，将随机卷曲变成了α螺旋。11 株中有 6 株的蛋白糖基化位点发生突变，从 NTTD 突变为 NITD。此外，流行病学数据表明，TMUV 已经在中国一半的省份（34 个中的 17 个）流行。我们的发现首次确定 NS1 蛋白是遗传进化的潜在高变区。此外，病毒的地域范围已经扩大，需要严格的生物安全措施或多价疫苗来控制其传播。
