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Tn5编码的链霉素抗性在大肠杆菌中的表达。

Expression of Tn5-encoded streptomycin resistance in E. coli.

作者信息

Mazodier P, Genilloud O, Giraud E, Gasser F

出版信息

Mol Gen Genet. 1986 Sep;204(3):404-9. doi: 10.1007/BF00331016.

DOI:10.1007/BF00331016
PMID:3020378
Abstract

Four Tn5 mutations able to express streptomycin resistance in E. coli were obtained independently. These mutations (called Tn5) were localized and sequenced. All of them consist of a 6 bp deletion in the str gene near the 3' end. The mutation affects a region peculiar for its repetition of an identical 6 bp sequence. The mutation does not affect the level of transcription of the kan, ble, str operon of Tn5, neither does it increase the level of translation of str. The mutation seems to interfere with a post-translational event.

摘要

独立获得了四个能在大肠杆菌中表达链霉素抗性的Tn5突变体。对这些突变(称为Tn5)进行了定位和测序。它们均由3'端附近str基因中的6 bp缺失组成。该突变影响一个因其重复相同6 bp序列而独特的区域。该突变不影响Tn5的kan、ble、str操纵子的转录水平,也不增加str的翻译水平。该突变似乎干扰了翻译后事件。

相似文献

1
Expression of Tn5-encoded streptomycin resistance in E. coli.Tn5编码的链霉素抗性在大肠杆菌中的表达。
Mol Gen Genet. 1986 Sep;204(3):404-9. doi: 10.1007/BF00331016.
2
A clinical isolate of transposon Tn5 expressing streptomycin resistance in Escherichia coli.在大肠杆菌中表达链霉素抗性的转座子Tn5的临床分离株。
J Bacteriol. 1988 Mar;170(3):1275-8. doi: 10.1128/jb.170.3.1275-1278.1988.
3
Tn5 carries a streptomycin resistance determinant downstream from the kanamycin resistance gene.Tn5在卡那霉素抗性基因下游携带一个链霉素抗性决定簇。
Mol Gen Genet. 1983;191(2):288-94. doi: 10.1007/BF00334828.
4
Bleomycin increases amikacin and streptomycin resistance in Escherichia coli harboring transposon Tn5.博来霉素可增加携带转座子Tn5的大肠杆菌对阿米卡星和链霉素的耐药性。
Antimicrob Agents Chemother. 1993 Sep;37(9):1982-5. doi: 10.1128/AAC.37.9.1982.
5
The transposon Tn5 carries a bleomycin-resistance determinant.转座子Tn5携带一个博来霉素抗性决定簇。
Gene. 1984 Dec;32(1-2):225-33. doi: 10.1016/0378-1119(84)90050-7.
6
Transposon Tn5 specifies streptomycin resistance in Rhizobium spp.转座子Tn5赋予根瘤菌属链霉素抗性。
J Bacteriol. 1984 May;158(2):580-9. doi: 10.1128/jb.158.2.580-589.1984.
7
Host-dependent transposon Tn5-mediated streptomycin resistance.宿主依赖性转座子Tn5介导的链霉素抗性。
J Bacteriol. 1984 Jul;159(1):395-9. doi: 10.1128/jb.159.1.395-399.1984.
8
Completion of the nucleotide sequence of the central region of Tn5 confirms the presence of three resistance genes.Tn5中心区域核苷酸序列的完成证实了三个抗性基因的存在。
Nucleic Acids Res. 1985 Jan 11;13(1):195-205. doi: 10.1093/nar/13.1.195.
9
Bleomycin-resistance gene derived from the transposon Tn5 confers selective advantage to Escherichia coli K-12.源自转座子Tn5的博来霉素抗性基因赋予大肠杆菌K-12选择性优势。
Proc Natl Acad Sci U S A. 1991 Oct 15;88(20):9112-6. doi: 10.1073/pnas.88.20.9112.
10
A restriction enzyme cleavage map of Tn5 and location of a region encoding neomycin resistance.Tn5的限制性内切酶切割图谱及编码新霉素抗性区域的位置。
Mol Gen Genet. 1979;177(1):65-72. doi: 10.1007/BF00267254.

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Mutation of the gidB gene causes intrinsic streptomycin resistance in Bacillus velezensis.gidB基因突变导致贝莱斯芽孢杆菌产生内在性链霉素抗性。
Sci Rep. 2025 Feb 15;15(1):5565. doi: 10.1038/s41598-025-90258-y.
2
Transiently silent acquired antimicrobial resistance: an emerging challenge in susceptibility testing.获得性抗菌药物耐药性的一过性沉默:药敏试验中一个新出现的挑战。
J Antimicrob Chemother. 2023 Mar 2;78(3):586-598. doi: 10.1093/jac/dkad024.
3
Use of a transposon with luciferase as a reporter to identify environmentally responsive genes in a cyanobacterium.

本文引用的文献

1
Aminoglycoside uptake and mode of action--with special reference to streptomycin and gentamicin. I. Antagonists and mutants.氨基糖苷类药物的摄取及作用方式——特别提及链霉素和庆大霉素。I. 拮抗剂与突变体。
J Antimicrob Chemother. 1981 Oct;8(4):249-76. doi: 10.1093/jac/8.4.249.
2
Host-dependent transposon Tn5-mediated streptomycin resistance.宿主依赖性转座子Tn5介导的链霉素抗性。
J Bacteriol. 1984 Jul;159(1):395-9. doi: 10.1128/jb.159.1.395-399.1984.
3
Transposon Tn5 encodes streptomycin resistance in nonenteric bacteria.转座子Tn5在非肠道细菌中编码链霉素抗性。
使用带有荧光素酶作为报告基因的转座子来鉴定蓝细菌中的环境响应基因。
Proc Natl Acad Sci U S A. 1991 Jun 15;88(12):5355-9. doi: 10.1073/pnas.88.12.5355.
4
Bleomycin increases amikacin and streptomycin resistance in Escherichia coli harboring transposon Tn5.博来霉素可增加携带转座子Tn5的大肠杆菌对阿米卡星和链霉素的耐药性。
Antimicrob Agents Chemother. 1993 Sep;37(9):1982-5. doi: 10.1128/AAC.37.9.1982.
5
Improved expression of streptomycin resistance in plants due to a deletion in the streptomycin phosphotransferase coding sequence.由于链霉素磷酸转移酶编码序列中的缺失,植物中链霉素抗性表达得到改善。
Mol Gen Genet. 1988 Nov;214(3):456-9. doi: 10.1007/BF00330480.
6
A clinical isolate of transposon Tn5 expressing streptomycin resistance in Escherichia coli.在大肠杆菌中表达链霉素抗性的转座子Tn5的临床分离株。
J Bacteriol. 1988 Mar;170(3):1275-8. doi: 10.1128/jb.170.3.1275-1278.1988.
7
Temporal control of transposition in Tn5.Tn5中转座的时间控制。
J Bacteriol. 1988 Feb;170(2):889-94. doi: 10.1128/jb.170.2.889-894.1988.
8
Bleomycin-kanamycin resistance as a marker of the presence of transposon Tn5 in clinical strains of Escherichia coli.博来霉素-卡那霉素抗性作为转座子Tn5在大肠杆菌临床菌株中存在的标志物。
Eur J Clin Microbiol Infect Dis. 1989 Nov;8(11):995-8. doi: 10.1007/BF01967573.
9
The bleomycin resistance gene of transposon Tn5 is an excellent marker for transformation of corynebacteria.转座子Tn5的博来霉素抗性基因是棒状杆菌转化的优良标记。
Appl Microbiol Biotechnol. 1992 Mar;36(6):759-62. doi: 10.1007/BF00172189.
J Bacteriol. 1984 Jul;159(1):388-9. doi: 10.1128/jb.159.1.388-389.1984.
4
Tn5 carries a streptomycin resistance determinant downstream from the kanamycin resistance gene.Tn5在卡那霉素抗性基因下游携带一个链霉素抗性决定簇。
Mol Gen Genet. 1983;191(2):288-94. doi: 10.1007/BF00334828.
5
Nucleotide sequence and exact localization of the neomycin phosphotransferase gene from transposon Tn5.转座子Tn5中新霉素磷酸转移酶基因的核苷酸序列及精确定位。
Gene. 1982 Oct;19(3):327-36. doi: 10.1016/0378-1119(82)90023-3.
6
Evidence for two functional gal promoters in intact Escherichia coli cells.完整大肠杆菌细胞中两个功能性半乳糖启动子的证据。
J Biol Chem. 1981 Nov 25;256(22):11905-10.
7
Structural analysis of Tn5.Tn5的结构分析
Cold Spring Harb Symp Quant Biol. 1981;45 Pt 1:107-13. doi: 10.1101/sqb.1981.045.01.019.
8
The functional differences in the inverted repeats of Tn5 are caused by a single base pair nonhomology.Tn5反向重复序列中的功能差异是由单个碱基对的非同源性引起的。
Cell. 1981 Jan;23(1):191-9. doi: 10.1016/0092-8674(81)90284-1.
9
Sequencing end-labeled DNA with base-specific chemical cleavages.通过碱基特异性化学切割对末端标记的DNA进行测序。
Methods Enzymol. 1980;65(1):499-560. doi: 10.1016/s0076-6879(80)65059-9.
10
The inverted repeats of Tn5 are functionally different.转座子Tn5的反向重复序列在功能上有所不同。
Cell. 1980 Mar;19(3):795-805. doi: 10.1016/s0092-8674(80)80055-9.