Department of Clinical and Experimental Medicine, University of Foggia, Via Rovelli 50, Foggia 71122, Italy.
Maxillofacial and ENT Surgery Department, Istituto Nazionale per lo Studio e la Cura dei Tumori, Fondazione G. Pascale, IRCCS, Naples 80131, Italy.
Int J Mol Sci. 2018 Sep 8;19(9):2664. doi: 10.3390/ijms19092664.
Survivin is a well-known protein involved in the inhibition of apoptosis in many different cancer types. The aim of this study was to perform an integrated bioinformatic and histologic analysis in order to study the expression and prognostic role of Survivin and its related gene in oral cancer. Publicly available databases were accessed via Gene Expression Omnibus and Oncomine, in addition raw data from The Cancer Genome Atlas (TCGA) were also obtained in order to analyze the rate of gene mutation, expression and methylation in patients with oral squamous cells carcinoma (OSCC). Immunohistochemistry (IHC) was also performed in order to evaluate the nuclear and cytoplasmic expression of Survivin and their correlation with cell proliferation in samples from OSCC patients. Results of this study revealed that Survivin is rarely mutated in OSCC samples and upregulated when compared to non-cancerous tissue. A negative correlation between the methylation of the island cg25986496 and BIRC5 mRNA expression was detected from TCGA data. IHC staining revealed that cytoplasmic (and not nuclear) expression of Survivin is associated with poor overall survival in OSCC patients, while the nuclear expression correlates with higher proliferation rate. In addition, data from TCGA database revealed that BIRC5 gene expression is an independent prognostic factor for OSCC patients.
Survivin 是一种在多种不同癌症类型中抑制细胞凋亡的知名蛋白。本研究的目的是通过综合生物信息学和组织学分析来研究 Survivin 及其相关基因在口腔癌中的表达和预后作用。通过基因表达综合数据库（Gene Expression Omnibus）和 Oncomine 访问了公开可用的数据库，此外还从癌症基因组图谱（The Cancer Genome Atlas，TCGA）获取了原始数据，以分析口腔鳞状细胞癌（OSCC）患者的基因突变、表达和甲基化率。还进行了免疫组织化学（Immunohistochemistry，IHC），以评估 Survivin 的核和细胞质表达及其与 OSCC 患者样本中细胞增殖的相关性。本研究结果表明，Survivin 在 OSCC 样本中很少发生突变，与非癌组织相比表达上调。从 TCGA 数据中检测到岛 cg25986496 的甲基化与 BIRC5 mRNA 表达呈负相关。IHC 染色显示，Survivin 的细胞质（而非核）表达与 OSCC 患者的总生存率降低相关，而核表达与更高的增殖率相关。此外，TCGA 数据库的数据表明，BIRC5 基因表达是 OSCC 患者的独立预后因素。
Zotero 插件
