Org Lett. 2018 Sep 21;20(18):5614-5617. doi: 10.1021/acs.orglett.8b02296. Epub 2018 Sep 12.
Cyclopropenethiones are reported as new bioorthogonal reagents. These motifs react readily with substituted phosphines to provide thiocarbonyl adducts. In some cases, the ligations are >300-fold faster than analogous reactions with bioorthogonal cyclopropenones. Dialkyl cyclopropenethiones are also stable in aqueous buffers and can be used for biomolecule labeling in vitro and in cell lysate. The rapid reactivity and biocompatibility of cyclopropenethiones suggest that they will be useful probes for cellular studies.
环丙叉硫酮被报道为新的生物正交试剂。这些基团可与取代膦快速反应，生成硫羰加合物。在某些情况下，这些连接反应的速度比类似的生物正交环丙酮反应快 300 多倍。二烷基环丙叉硫酮在水缓冲液中也很稳定，可用于体外和细胞裂解物中的生物分子标记。环丙叉硫酮的快速反应性和生物相容性表明，它们将成为细胞研究的有用探针。
