Poehlein Anja, Schneider Dominik, Soh Melissa, Daniel Rolf, Seedorf Henning
Genomic and Applied Microbiology and Göttingen Genomics Laboratory, Georg-August University, Göttingen, Germany.
1 Research Link, Temasek Life Sciences Laboratory, Singapore 117604.
Archaea. 2018 Aug 19;2018:7609847. doi: 10.1155/2018/7609847. eCollection 2018.
and species represent some of the most prevalent methanogenic archaea in the gastrointestinal tract of animals and humans and play an important role in this environment. The aim of this study was to identify genomic features that are shared or specific for members of each genus with a special emphasis of the analysis on the assimilation of nitrogen and acetate and the utilization of methanol and ethanol for methanogenesis. Here, draft genome sequences of strain DSM 11995, strain DSM 11979, and strain 4103 are reported and compared to those of 16 other and genomes, including genomes of the 13 currently available types of strains of the two genera. The comparative genome analyses indicate that among other genes, the absence of molybdopterin cofactor biosynthesis is conserved in species but reveals also that the three species share a core set of more than 300 genes that distinguishes the genus from the genus . Multilocus sequence analysis shows that the genus can be subdivided into clades, potentially new genera, which may display characteristic specific metabolic features. These features include not only the potential ability of nitrogen fixation and acetate assimilation in a clade comprised of species from the termite gut and strains but also the potential capability to utilize ethanol and methanol in a clade comprising strain DSM 11976, sp. AbM4, and strain DSM 25824.
甲烷短杆菌属物种是动物和人类胃肠道中一些最普遍的产甲烷古菌，在这种环境中发挥着重要作用。本研究的目的是确定每个属成员共有的或特有的基因组特征，特别着重于对氮和乙酸同化以及利用甲醇和乙醇进行甲烷生成的分析。在此，报告了菌株DSM 11995、菌株DSM 11979和菌株4103的基因组草图序列，并与其他16个甲烷短杆菌属和甲烷杆菌属基因组进行了比较，包括两个属目前可用的13种菌株类型的基因组。比较基因组分析表明，在其他基因中，钼蝶呤辅因子生物合成的缺失在甲烷短杆菌属物种中是保守的，但也揭示了这三个物种共享一组超过300个基因的核心集，这将甲烷短杆菌属与甲烷杆菌属区分开来。多位点序列分析表明，甲烷短杆菌属可细分为进化枝，可能是新的属，它们可能表现出特征性的特定代谢特征。这些特征不仅包括在由来自白蚁肠道的甲烷短杆菌属物种和菌株组成的进化枝中固氮和乙酸同化的潜在能力，还包括在由菌株DSM 11976、AbM4甲烷短杆菌和菌株DSM 25824组成的进化枝中利用乙醇和甲醇的潜在能力。
