Poehlein Anja, Daniel Rolf, Seedorf Henning
Genomic and Applied Microbiology & Göttingen Genomics Laboratory, Georg-August University, Göttingen, Germany.
Temasek Life Sciences Laboratory, Singapore.
Archaea. 2017 May 28;2017:4097425. doi: 10.1155/2017/4097425. eCollection 2017.
strain DH1 is an autotrophic methanogen that was isolated from the wetwood of methane-emitting trees. This species has been of considerable interest for its unusual oxygen tolerance and has been studied as a model organism for more than four decades. Strain DH1 is closely related to other host-associated species from intestinal tracts of animals and the rumen, making this strain an interesting candidate for comparative analysis to identify factors important for colonizing intestinal environments. Here, the genome sequence of strain DH1 is reported. The draft genome is composed of 2.445.031 bp with an average GC content of 25.44% and predicted to harbour 1964 protein-encoding genes. Among the predicted genes, there are also more than 50 putative genes for the so-called adhesin-like proteins (ALPs). The presence of ALP-encoding genes in the genome of this non-host-associated methanogen strongly suggests that target surfaces for ALPs other than host tissues also need to be considered as potential interaction partners. The high abundance of ALPs may also indicate that these types of proteins are more characteristic for specific phylogenetic groups of methanogens rather than being indicative for a particular environment the methanogens thrives in.
菌株DH1是一种自养型产甲烷菌，从排放甲烷的树木的湿木中分离得到。该物种因其不同寻常的耐氧性而备受关注，并且作为模式生物已被研究了四十多年。菌株DH1与来自动物肠道和瘤胃的其他宿主相关物种密切相关，这使得该菌株成为进行比较分析以确定在肠道环境定殖中重要因素的有趣候选对象。在此，报道了菌株DH1的基因组序列。基因组草图由2,445,031个碱基对组成，平均GC含量为25.44%，预计含有1964个蛋白质编码基因。在预测的基因中，还有50多个所谓的类粘附素蛋白（ALP）的推定基因。在这种非宿主相关产甲烷菌的基因组中存在编码ALP的基因，强烈表明除宿主组织外，ALP的靶表面也需要被视为潜在的相互作用伙伴。ALP的高丰度也可能表明这些类型的蛋白质对于产甲烷菌的特定系统发育组更具特征性，而不是指示产甲烷菌所生长的特定环境。
