Rahman Zillur, Rahman Md Anisur, Rashid Mahamud-Ur, Monira Shirajum, Johura Fatema-Tuz, Mustafiz Munshi, Bhuyian Sazzadul I, Zohura Fatema, Parvin Tahmina, Hasan Khaled, Saif-Ur-Rahman K M, Islam Nazneen N, Sack David A, George Christine M, Alam Munirul
Infectious Diseases Division, International Center for Diarrhoeal Disease Research, Dhaka, Bangladesh.
Department of Biotechnology and Genetic Engineering, Noakhali Science and Technology University, Noakhali, Bangladesh.
Front Public Health. 2018 Aug 30;6:238. doi: 10.3389/fpubh.2018.00238. eCollection 2018.
Recurrent cholera causes significant morbidity and mortality in cholera endemic estuarine areas of Bangladesh. There have been limited studies to investigate the transmission patterns of associated with cholera in Bangladesh. In this study, we characterized serogroup O1 isolated from 30 cholera patients, 76 household contacts, 119 stored drinking water samples, and 119 water source samples in Bakerganj and Mathbaria, two cholera endemic coastal regions in Bangladesh. Results of phenotypic and molecular characterization of isolates ( = 56) confirmed them to be toxigenic belonging to serogroup O1 biotype El Tor (ET), and possessing cholera toxin of the classical biotype (altered ET). Molecular fingerprinting of the O1 of clinical and water origins determined by PFGE of -I- digested genomic DNA showed them to be closely related, as the PFGE banding patterns were highly homogenous. Phylogenetic analysis using dendrogram of cholera patients, household contacts, and household groundwater sources showed isolates within households to be clonally linked, suggesting water as an important vehicle of transmission of cholera in the coastal villages of Bangladesh. Transmission of toxigenic O1 through drinking water in cholera endemic rural settings underscores the urgent need for evidence based water, sanitation, and hygiene interventions promoting safe drinking water to prevent morbidity and mortality related to cholera and other enteric infections in Bangladesh.
复发性霍乱在孟加拉国霍乱流行的河口地区导致了显著的发病率和死亡率。在孟加拉国，针对与霍乱相关的传播模式进行调查的研究有限。在本研究中，我们对从孟加拉国两个霍乱流行的沿海地区——贝克甘杰和马图巴里的30名霍乱患者、76名家庭接触者、119份储存饮用水样本以及119份水源样本中分离出的O1血清群霍乱弧菌进行了特征分析。对56株分离株进行表型和分子特征分析的结果证实它们产毒，属于O1血清群埃尔托生物型（ET），并拥有经典生物型的霍乱毒素（变异型ET）。通过对经I酶切的基因组DNA进行脉冲场凝胶电泳（PFGE）确定的临床和水源O1霍乱弧菌的分子指纹图谱显示它们密切相关，因为PFGE条带模式高度一致。使用霍乱患者、家庭接触者和家庭地下水源的树状图进行系统发育分析表明，家庭中的分离株存在克隆关联，这表明水是孟加拉国沿海村庄霍乱传播的重要媒介。在霍乱流行的农村地区，产毒O1霍乱弧菌通过饮用水传播，这突出表明迫切需要基于证据的水、环境卫生和个人卫生干预措施，以推广安全饮用水，预防孟加拉国与霍乱和其他肠道感染相关的发病率和死亡率。
