Miyahara M, Maruyama T, Wake A, Mise K
Department of Microbiology, National Institute of Hygienic Sciences, Tokyo, Japan.
Appl Environ Microbiol. 1988 Feb;54(2):577-80. doi: 10.1128/aem.54.2.577-580.1988.
The cold-active restriction endonuclease YenI, an isoschizomer of PstI, was found in 12 of 14 Yersinia enterocolitica serotype O8 strains of different origins, but not in other serotypes of Y. enterocolitica, Yersinia pseudotuberculosis, or Yersinia pestis. In spite of the limited number of strains tested, the result suggests that the detection of YenI endonuclease or the gene might result in more rapid determination of the prominently pathogenic serotype of Y. enterocolitica.
冷活性限制性内切酶YenI是PstI的同裂酶,在14株不同来源的小肠结肠炎耶尔森菌O8血清型菌株中的12株中被发现,但在小肠结肠炎耶尔森菌的其他血清型、假结核耶尔森菌或鼠疫耶尔森菌中未发现。尽管测试的菌株数量有限,但该结果表明,检测YenI内切酶或其基因可能会更快速地确定小肠结肠炎耶尔森菌的主要致病血清型。