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甘蓝型油菜 BOR 转运蛋白基因家族的全基因组分子特征及 BnaC04.BOR1;1c 的遗传分析。

Molecular characterization of the genome-wide BOR transporter gene family and genetic analysis of BnaC04.BOR1;1c in Brassica napus.

机构信息

National Key Laboratory of Crop Genetic Improvement, Microelement Research Center, Huazhong Agricultural University, Wuhan, 430070, China.

出版信息

BMC Plant Biol. 2018 Sep 14;18(1):193. doi: 10.1186/s12870-018-1407-1.

DOI:10.1186/s12870-018-1407-1
PMID:30217178
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6137915/
Abstract

BACKGROUND

Boron (B) deficiency is an agricultural problem that causes significant losses of crop yield in many areas of the world. However, systematic analysis of BOR family genes for B transport in rapeseed is still lacking. We aimed to identify and characterize BOR transporters in Brassica napus and the potential role of these transporters in B homeostasis in response to B deficiency.

RESULTS

Here, we identified 20 BOR transporters from the Brassica napus genome, which were classified into six distinct groups that represent clear orthologous relationships to their family members in Arabidopsis. qRT-PCR revealed distinct expression profiles for BnBORs in different tissues and in response to external B levels. The B-efficient cultivar QY10 accumulated more B in shoots than the B-inefficient cultivar W10, and overexpression of BnaBOR1;1c could alleviate shoot B-deficiency symptoms in W10 by distributing more B from roots to shoots. Additionally, BnBOR1;1c expression was up-regulated by B deficiency, and the induction of BnBOR1;1c was more intense in QY10. Moreover, two conserved InDels were found in the promoter regions of BnBOR1;1c within different B-efficient genotypes.

CONCLUSIONS

Overall, the molecular characterization of the BnBOR genes of two B-efficient cultivars and their responses to B deficiency highlights the diversity of the family members in B. napus, and BnaC4.BOR1;1c has potential as a candidate gene for improving B nutrition.

摘要

背景

硼（B）缺乏是一个农业问题，它导致世界上许多地区的作物产量显著损失。然而，油菜中 BOR 家族基因对 B 运输的系统分析仍然缺乏。我们的目的是鉴定和描述油菜中 BOR 转运蛋白，以及这些转运蛋白在应对 B 缺乏时对 B 体内平衡的潜在作用。

结果

在这里，我们从油菜基因组中鉴定出 20 个 BOR 转运蛋白，它们分为六个不同的组，代表与拟南芥家族成员明显的同源关系。qRT-PCR 揭示了 BnBORs 在不同组织和对外界 B 水平响应中的不同表达谱。B 高效品种 QY10 在地上部分积累的 B 比 B 低效品种 W10 多，并且过表达 BnaBOR1;1c 可以通过将更多的 B 从根部分配到地上部分来缓解 W10 地上部分的 B 缺乏症状。此外，B 缺乏会诱导 BnBOR1;1c 的表达，并且在 QY10 中 BnBOR1;1c 的诱导更为强烈。此外，在不同 B 高效基因型的 BnBOR1;1c 启动子区域发现了两个保守的 InDels。

结论

总之，对两个 B 高效品种的 BnBOR 基因的分子特征及其对 B 缺乏的响应突出了 B. napus 中家族成员的多样性，并且 BnaC4.BOR1;1c 具有作为改善 B 营养的候选基因的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/044b/6137915/d547e2797dfe/12870_2018_1407_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/044b/6137915/aec6e6580039/12870_2018_1407_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/044b/6137915/96e48b3871a8/12870_2018_1407_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/044b/6137915/298738ca72e0/12870_2018_1407_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/044b/6137915/84e9f65c83b1/12870_2018_1407_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/044b/6137915/1c224591859a/12870_2018_1407_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/044b/6137915/d19163b0ae89/12870_2018_1407_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/044b/6137915/d655511fe947/12870_2018_1407_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/044b/6137915/64c77036c66d/12870_2018_1407_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/044b/6137915/d547e2797dfe/12870_2018_1407_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/044b/6137915/aec6e6580039/12870_2018_1407_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/044b/6137915/96e48b3871a8/12870_2018_1407_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/044b/6137915/298738ca72e0/12870_2018_1407_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/044b/6137915/84e9f65c83b1/12870_2018_1407_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/044b/6137915/1c224591859a/12870_2018_1407_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/044b/6137915/d19163b0ae89/12870_2018_1407_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/044b/6137915/d655511fe947/12870_2018_1407_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/044b/6137915/64c77036c66d/12870_2018_1407_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/044b/6137915/d547e2797dfe/12870_2018_1407_Fig9_HTML.jpg

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