Janssen H P, van Loon A M, Meddens M J, Eickmans-Josten E C, Hoitsma A J, de Witte T J, Quint W G
Department of Medical Microbiology, University of Nijmegen, The Netherlands.
J Clin Microbiol. 1988 Jul;26(7):1313-5. doi: 10.1128/jcm.26.7.1313-1315.1988.
Detection methods for human cytomegalovirus were evaluated with 431 urine samples from 30 bone marrow and 88 kidney transplant recipients. Low-speed centrifugal inoculation was followed by early antigen (EA) detection by means of indirect immunofluorescence with a monoclonal antibody after 1 (EA-1) and 6 (EA-6) days of cultivation. The results were compared with those of conventional virus isolation (CVI). Of 68 positive samples, 49 (72%) were detected with EA-1, 58 (85%) were detected with EA-6, and 43 (63%) were detected with CVI. The combination of EA-1 and EA-6 showed positive results with 66 samples (97%), which is significantly better than with CVI (P less than 0.001). With the exception of one patient, all CVI-negative but EA-positive samples had either significant rises in immunoglobulin G (IgG) or IgA antibody titer or IgM antibodies present in the sera. These data indicate that the method with EA detection can replace CVI, provided that each sample is inoculated in duplicate. Sample 1 is examined after 1 day, and if it is negative, sample 2 is incubated for a further 5 days, followed by detection of cytomegalovirus.
采用来自30名骨髓移植受者和88名肾移植受者的431份尿液样本，对人巨细胞病毒的检测方法进行了评估。采用低速离心接种，接种后分别于培养1天（EA-1）和6天（EA-6）通过单克隆抗体间接免疫荧光法检测早期抗原（EA）。将结果与传统病毒分离法（CVI）的结果进行比较。在68份阳性样本中，EA-1检测出49份（72%），EA-6检测出58份（85%），CVI检测出43份（63%）。EA-1和EA-6联合检测对66份样本（97%）呈阳性结果，显著优于CVI（P小于0.001）。除1例患者外，所有CVI阴性但EA阳性的样本，其血清中免疫球蛋白G（IgG）或IgA抗体滴度显著升高，或存在IgM抗体。这些数据表明，只要每个样本接种两份，EA检测法可替代CVI。样本1在1天后进行检测，若为阴性，则样本2再培养5天，随后检测巨细胞病毒。
