Hajighasem Amir, Farzanegi Parvin, Mazaheri Zohreh, Naghizadeh Marjan, Salehi Ghoncheh
Department of Exercise Physiology, Sari Branch, Islamic Azad University, Sari, Iran.
Department of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
PeerJ. 2018 Sep 10;6:e5522. doi: 10.7717/peerj.5522. eCollection 2018.
Non-alcoholic fatty liver disease (NAFLD) is the most common chronic liver disorder. This study aims to consider effects of resveratrol, exercise and their combination on genes expression in the liver of elderly rats with NAFLD.
Rats with NAFLD were randomly divided into seven groups including patient, saline, resveratrol (RSV), interval exercise, continuous exercise, interval exercise + RSV and continuous exercise + RSV. Levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) in the liver tissue were measured using specific ELISA kits. A TUNEL assay kit was used for the assessment of hepatic cells apoptosis. Lipid profiles were considered by measuring the serum triglyceride, cholesterol, LDL, and HDL. Expression of , and genes was considered using RT-PCR.
Resveratrol administration alone or combined with exercise training significantly improved the expression of , and genes ( < 0.05) in the hepatic tissue of rats with NAFLD, while levels of AST, ALT, ALP enzymes, as well as apoptotic cells were significantly decreased ( < 0.05).
Although resveratrol alone improves the expression of , and , as well as liver function, combined therapy with exercise training is more effective to improve NAFLD.
非酒精性脂肪性肝病（NAFLD）是最常见的慢性肝脏疾病。本研究旨在探讨白藜芦醇、运动及其联合应用对老年NAFLD大鼠肝脏基因表达的影响。
将NAFLD大鼠随机分为七组，包括模型组、生理盐水组、白藜芦醇（RSV）组、间歇运动组、持续运动组、间歇运动+RSV组和持续运动+RSV组。使用特定的ELISA试剂盒检测肝组织中丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）和碱性磷酸酶（ALP）的水平。使用TUNEL检测试剂盒评估肝细胞凋亡情况。通过测量血清甘油三酯、胆固醇、低密度脂蛋白和高密度脂蛋白来分析血脂情况。使用RT-PCR检测 、 和 基因的表达。
单独给予白藜芦醇或与运动训练联合应用均能显著改善NAFLD大鼠肝组织中 、 和 基因的表达（P<0.05），同时AST、ALT、ALP酶水平以及凋亡细胞数量均显著降低（P<0.05）。
虽然单独使用白藜芦醇可改善 、 和 的表达以及肝功能，但运动训练联合治疗对改善NAFLD更有效。
