Department of Biological Science, Xi'an Jiaotong‑Liverpool University, Suzhou, Jiangsu 215123, P.R. China.
Department of Thoracic Surgery, Yan'an Hospital Affiliated to Kunming Medical University, Kunming, Yunnan 650051, P.R. China.
Mol Med Rep. 2018 Nov;18(5):4725-4732. doi: 10.3892/mmr.2018.9463. Epub 2018 Sep 6.
Long noncoding RNA LINC00978 has been reported to regulate the progression of several human types of cancer, including gastric and breast cancer. However, knowledge on LINC00978 in non‑small cell lung cancer (NSCLC) is limited. In the present study, it was demonstrated that LINC00978 expression was significantly upregulated in NSCLC tissues compared with the adjacent normal tissues. Furthermore, LINC00978 expression was positively correlated with the tumor, node and metastasis stage, and lymph node metastasis in NSCLC patients. Additionally, LINC00978 knockdown significantly inhibited the proliferation, migration and invasion of NSCLC cells while promoting cell apoptosis. In terms of the underlying mechanism, it was demonstrated that LINC00978 served as a competing endogenous RNA sponge for microRNA (miR)‑6754‑5p, which was downregulated in NSCLC tissues. The present study demonstrated that there was a negative correlation between LINC00978 and miR‑6754‑5p expression levels in NSCLC tissues. Additionally, it was demonstrated that inhibition of miR‑6754‑5p reversed the effects of LINC00978 knockdown on NSCLC cell proliferation, migration, invasion and apoptosis. In conclusion, results of the present study demonstrated that LINC00978 exerts an oncogenic role in NSCLC by inhibiting miR‑6754‑5p expression.
长链非编码 RNA LINC00978 已被报道可调节多种人类类型的癌症的进展，包括胃癌和乳腺癌。然而，LINC00978 在非小细胞肺癌（NSCLC）中的知识有限。在本研究中，证明与相邻正常组织相比，LINC00978 在 NSCLC 组织中表达明显上调。此外，LINC00978 的表达与 NSCLC 患者的肿瘤、淋巴结和转移分期以及淋巴结转移呈正相关。此外，LINC00978 敲低显著抑制 NSCLC 细胞的增殖、迁移和侵袭，同时促进细胞凋亡。就潜在机制而言，证明 LINC00978 作为 microRNA（miR）-6754-5p 的竞争性内源性 RNA 海绵，miR-6754-5p 在 NSCLC 组织中下调。本研究表明，LINC00978 和 miR-6754-5p 在 NSCLC 组织中的表达水平呈负相关。此外，还证明抑制 miR-6754-5p 可逆转 LINC00978 敲低对 NSCLC 细胞增殖、迁移、侵袭和凋亡的影响。总之，本研究结果表明，LINC00978 通过抑制 miR-6754-5p 的表达在 NSCLC 中发挥致癌作用。
