Cheptsov V S, Churbanova E S, Yusupov V I, Gorlenko M V, Lysak L V, Minaev N V, Bagratashvili V N, Chichkov B N
Soil Science Faculty, Lomonosov Moscow State University, Moscow, Russia.
Institute of Photon Technologies, FSRC "Crystallography and Photonics", RAS, Moscow, Russia.
Lett Appl Microbiol. 2018 Dec;67(6):544-549. doi: 10.1111/lam.13074. Epub 2018 Oct 18.
Recently, it was shown that laser-induced forward transfer (LIFT) technology and the laser engineering of microbial systems (LEMS) technique (based on LIFT method) are effective for isolation of micro-organisms from different complex substrates. These techniques frequently utilize Au as an absorbing layer material. The purpose of this study was to investigate the influence of absorbing film materials (Au, Ti and Cr) on the effectiveness of laser printing of micro-organisms to improve LEMS and LIFT techniques. It was shown that application of Ti and Cr absorbing layers activates bacterial growth after laser printing and is significantly more effective in comparison to Au films, which actually show a suppressing effect on bacterial cells. Results of this study can be applied for LEMS and LIFT protocols for improving bacterial isolation and microbial growth. SIGNIFICANCE AND IMPACT OF THE STUDY: Laser-induced forward transfer technique (LIFT) is currently used for printing of micro-organisms and in biosensor techniques, for single-cell isolation, and for culturing of micro-organisms from complex substrates. We have studied the influence of absorbing film materials (Au, Ti and Cr) on the effectiveness laser printing of micro-organisms. It was shown that application of Ti and Cr absorbing layers activates bacterial growth and is more effective in LIFT compared to Au films, which actually have a suppressive effect on bacteria cells. The results can improve LIFT protocols for bacteria isolation and culturing of microbial systems.
最近的研究表明，激光诱导正向转移（LIFT）技术以及基于LIFT方法的微生物系统激光工程（LEMS）技术，对于从不同复杂基质中分离微生物是有效的。这些技术经常使用金作为吸收层材料。本研究的目的是探究吸收膜材料（金、钛和铬）对微生物激光打印效果的影响，以改进LEMS和LIFT技术。研究表明，应用钛和铬吸收层可在激光打印后激活细菌生长，与实际上对细菌细胞有抑制作用的金膜相比，其效果显著更佳。本研究结果可应用于LEMS和LIFT方案，以改善细菌分离和微生物生长。研究的意义和影响：激光诱导正向转移技术（LIFT）目前用于微生物打印、生物传感器技术、单细胞分离以及从复杂基质中培养微生物。我们研究了吸收膜材料（金、钛和铬）对微生物激光打印效果的影响。研究表明，应用钛和铬吸收层可激活细菌生长，在LIFT中比金膜更有效，而金膜实际上对细菌细胞有抑制作用。这些结果可改进用于细菌分离和微生物系统培养的LIFT方案。
