Liaoning Province Key Laboratory of Plup and Papermaking Engineering, Dalian Polytech- nic University, Dalian, l16034, China.
Key Laboratory of Industrial Ecology and Environmental Engineering, China Ministry of Education, Dalian, 116024, China.
Sci Rep. 2018 Sep 17;8(1):13947. doi: 10.1038/s41598-018-32013-0.
Biochars produced from two different wood species over a microwave assisted pyrolysis process were used as novel and green-based supports for immobilizing enzyme, laccase in particular. The results obtained from FT-IR, SEM and BET measurements indicated that Maple biochar with honeycomb structure has higher surface area and pore volume than Spruce biochar; and there exist O-H, C-H, C=O and C=C groups in biochars for potential chemical modification. The best laccase immobilization conditions identified from an orthogonal experiment were pH = 3, laccase concentration 16 g/L and contact time 8 h. Under such conditions, the high immobilization yield (64.2%) and amount (11.14 mg/g) of laccase on Maple biochar were achieved, leading to the significantly improved thermal stability of laccase. Moreover, the immobilized laccase is reusable and enhanced the enzymatic degradation of 4-hydroxy-3,5-dichlorobiphenyl (71.4% yield), thus creating a promising and novel type of adsorbent in the removal of polychlorinated biphenyls from wastewater.
利用微波辅助热解工艺，将两种不同木材制备的生物炭作为新型绿色载体用于固定化酶（特别是漆酶）。FT-IR、SEM 和 BET 测量结果表明，具有蜂窝状结构的枫木生物炭比云杉生物炭具有更高的表面积和孔体积；生物炭中存在 O-H、C-H、C=O 和 C=C 基团，可用于潜在的化学修饰。通过正交实验确定的最佳漆酶固定化条件为 pH=3、漆酶浓度 16 g/L 和接触时间 8 h。在此条件下，枫木生物炭上漆酶的高固定化产率（64.2%）和量（11.14 mg/g）得以实现，从而显著提高了漆酶的热稳定性。此外，固定化漆酶可重复使用，并增强了对 4-羟基-3,5-二氯联苯的酶促降解（产率为 71.4%），从而为从废水中去除多氯联苯创造了一种有前景的新型吸附剂。
