Feighery C, Whelan C A, Weir D G, Greally J F
Clin Exp Immunol. 1978 Jun;32(3):459-65.
Peripheral blood mononuclear cells (PBMC) from normal donors, pre-cultured at 37 degrees C for 24 hr before the addition of mitogen, demonstrated an enhanced proliferative response. This may be due to the loss of a subpopulation of suppressor cells during the incubation period. Still further enhancement was observed when pre-culturing was prolonged for 48 hr, while cells pre-incubated at 4 degrees C showed no increased responsiveness. Concanavalin A (Con A) pre-activated PBMC supressed the mitogen response of responder cells. More marked suppression was observed when the concentration of Con A used to induce the suppressor cells was increased. It was not possible to activate suppressor function in cells which had been kept in vitro for longer than 48 hr. These findings support the concept of the existence and function of suppressor cells, and that the suppressive influence is short-lived in vitro culture.
来自正常供体的外周血单个核细胞（PBMC），在添加促有丝分裂原之前于37℃预培养24小时，表现出增强的增殖反应。这可能是由于在培养期间抑制细胞亚群的丢失。当预培养延长至48小时时，观察到进一步的增强，而在4℃预孵育的细胞未显示反应性增加。伴刀豆球蛋白A（Con A）预激活的PBMC抑制反应细胞的促有丝分裂原反应。当用于诱导抑制细胞的Con A浓度增加时，观察到更明显的抑制。对于在体外保存超过48小时的细胞，不可能激活其抑制功能。这些发现支持了抑制细胞的存在和功能的概念，以及这种抑制作用在体外培养中是短暂的。
