Department of General Surgery, the First Affiliated Hospital of Wenzhou Medical University, China.
Eur Rev Med Pharmacol Sci. 2018 Sep;22(17):5525-5530. doi: 10.26355/eurrev_201809_15813.
MicroRNAs (miRNAs) play critical roles in regulating tumor development and progression. The aim of the study is to investigate the clinical significance of miR-1294 expression in gastric cancer (GC).
The expression of miR-1294 in 82 cases of GC tissues and adjacent normal tissues was determined using quantitative Real Time-PCR (qRT-PCR) analyses. Survival plot was calculated using the Kaplan-Meier methods and log-rank test from the date of operation to the time of death or last follow-up date. The association between miR-1294 expression and clinical categorical data was analyzed using the chi-squared test. Moreover, Univariate and multivariate Cox analysis were performed to assess the risk factors of GC prognosis.
We showed that miR-1294 expression was significantly downregulated in GC tissues compared to adjacent normal tissues. The low expression of miR-1294 in patients with GC was correlated with clinicopathological parameters including larger tumor size, lymph node metastasis, and distant metastasis. Kaplan-Meier survival analysis showed that GC patients with lower miR-1294 expression exhibited a shorter disease-free survival (DFS) and overall survival (OS) time compared to those patients with higher miR-1294 expression. Multivariate Cox analysis showed that lower miR-1294 expression, tumor size, lymph node metastasis, and distant metastasis were identified as independent risk factors of GC prognosis.
Our results provided evidence that miR-1294 expression was significantly downregulated in GC and may serve as a predictor of GC prognosis.
MicroRNAs（miRNAs）在调控肿瘤发生和发展中发挥着关键作用。本研究旨在探讨 miR-1294 在胃癌（GC）中的表达的临床意义。
采用实时定量 PCR（qRT-PCR）分析检测 82 例胃癌组织和相邻正常组织中 miR-1294 的表达。采用 Kaplan-Meier 方法和对数秩检验从手术日期到死亡或最后随访日期计算生存曲线。采用卡方检验分析 miR-1294 表达与临床分类数据之间的关系。此外，还进行了单因素和多因素 Cox 分析，以评估 GC 预后的危险因素。
结果显示，miR-1294 在胃癌组织中的表达明显低于相邻正常组织。GC 患者 miR-1294 表达较低与肿瘤大小、淋巴结转移和远处转移等临床病理参数相关。Kaplan-Meier 生存分析显示，miR-1294 表达较低的 GC 患者无病生存（DFS）和总生存（OS）时间均短于 miR-1294 表达较高的患者。多因素 Cox 分析显示，miR-1294 表达较低、肿瘤大小、淋巴结转移和远处转移是 GC 预后的独立危险因素。
本研究结果表明，miR-1294 在 GC 中表达明显下调，可能作为 GC 预后的预测指标。
