Knight E, Hunkapiller M W, Korant B D, Hardy R W, Hood L E
Science. 1980 Feb 1;207(4430):525-6. doi: 10.1126/science.7352259.
The purification of human fibroblast interferon has been simplified to a two-step procedure consisting of affinity chromatography on Blue Sepharose and sodium dodecyl sulfate polyacrlamide gel electrophoresis. A preliminary amino acid composition and the sequence of the 13 amino-terminal residues of homogeneous interferon prepared by this method is reported.
人成纤维细胞干扰素的纯化已简化为两步程序,包括在蓝色琼脂糖凝胶上进行亲和层析以及十二烷基硫酸钠聚丙烯酰胺凝胶电泳。本文报道了用该方法制备的纯干扰素的初步氨基酸组成以及13个氨基末端残基的序列。