通过原位杂交对人白细胞、成纤维细胞和免疫干扰素基因进行染色体定位。
Chromosomal localization of human leukocyte, fibroblast, and immune interferon genes by means of in situ hybridization.
作者信息
Trent J M, Olson S, Lawn R M
出版信息
Proc Natl Acad Sci U S A. 1982 Dec;79(24):7809-13. doi: 10.1073/pnas.79.24.7809.
Abstract
Recombinant plasmids containing cDNA inserts from human leukocyte interferon (IFN-alpha), fibroblast interferon (IFN-beta), or immune interferon (INF-gamma) genes were radiolabeled and hybridized in situ to human metaphase chromosome preparations. The results localized the human IFN-alpha and IFN-beta genes to the short arm of chromosome 9(p21-->pter) and localized the IFN-gamma gene to the long arm of chromosome 12(q24.1).
摘要
含有来自人白细胞干扰素（IFN-α）、成纤维细胞干扰素（IFN-β）或免疫干扰素（IFN-γ）基因的互补DNA插入片段的重组质粒被放射性标记，并原位杂交到人类中期染色体标本上。结果将人IFN-α和IFN-β基因定位到9号染色体的短臂（p21→pter），并将IFN-γ基因定位到12号染色体的长臂（q24.1）。
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