Freedman R S, Bowen J M, Leibovitz A, Pathak S, Siciliano M J, Gallager H S, Giovanella B C
In Vitro. 1982 Aug;18(8):719-26. doi: 10.1007/BF02796428.
An established cell line, SW756, derived from a primary squamous carcinoma of the uterine cervix is described by its morphology, ultrastructure, karyotype, genetic signature analysis, HLA typing, and tumorigenesis in the nude mouse. Cultured cells obtained from the SW756 derived nude mouse tumor also were studied for chromosome and isozyme markers. The original tumor was poorly differentiated carcinoma with minimal keratinization and is compared with that occurring in the nude mouse after the cultured cells were inoculated. The nude mouse tumor showed similar histological features, but better differentiation than the original tumor. Karyotype analysis of SW756 demonstrated a hyperdiploid stem line number and several marker chromosomes (MI-M6). No HeLa marker chromosomes were identified. The isozyme pattern for SW756 reported by others has been confirmed. The unique chromosome and isozyme features have been identified repeatedly in the cultured cells and, most importantly, in the post nude mouse culture. We recommend SW756 as a defined human tumorigenic cell line derived from a primary squamous carcinoma of the uterine cervix.
描述了一种源自子宫颈原发性鳞状细胞癌的成熟细胞系SW756，包括其形态学、超微结构、核型、基因特征分析、HLA分型以及在裸鼠中的肿瘤发生情况。还对从SW756衍生的裸鼠肿瘤中获得的培养细胞进行了染色体和同工酶标记研究。原始肿瘤为低分化癌，角化极少，并与接种培养细胞后在裸鼠中发生的肿瘤进行了比较。裸鼠肿瘤显示出相似的组织学特征，但比原始肿瘤分化更好。SW756的核型分析显示超二倍体干细胞系数量和几条标记染色体（MI - M6）。未鉴定出HeLa标记染色体。其他人报道的SW756同工酶模式已得到证实。独特的染色体和同工酶特征在培养细胞中反复得到鉴定，最重要的是，在裸鼠培养后也得到了鉴定。我们推荐SW756作为一种源自子宫颈原发性鳞状细胞癌的明确的人致瘤细胞系。
