Shaw D J
J Med Genet. 1986 Oct;23(5):421-4. doi: 10.1136/jmg.23.5.421.
Recent advances in agarose gel electrophoresis of large DNA fragments raise the possibility of an entirely new approach to mapping mammalian genomes. In this article is discussed the potential of this technology for tackling problems such as construction of linkage maps, identifying chromosome translocation breakpoints, and moving from linked markers to genes causing diseases such as the muscular dystrophies and Huntington's chorea.
大DNA片段琼脂糖凝胶电泳技术的最新进展为绘制哺乳动物基因组图谱提供了全新的方法。本文探讨了该技术在解决诸如构建连锁图谱、识别染色体易位断点以及从连锁标记定位导致诸如肌肉萎缩症和亨廷顿舞蹈症等疾病的基因等问题方面的潜力。
