snmC-seq2 进行稳健的单细胞 DNA 甲基化组分析。

Genomic Analysis Laboratory, The Salk Institute for Biological Studies, La Jolla, CA, 92037, USA.

Howard Hughes Medical Institute, The Salk Institute for Biological Studies, La Jolla, CA, 92037, USA.

Nat Commun. 2018 Sep 20;9(1):3824. doi: 10.1038/s41467-018-06355-2.

Single-cell DNA methylome profiling has enabled the study of epigenomic heterogeneity in complex tissues and during cellular reprogramming. However, broader applications of the method have been impeded by the modest quality of sequencing libraries. Here we report snmC-seq2, which provides improved read mapping, reduced artifactual reads, enhanced throughput, as well as increased library complexity and coverage uniformity compared to snmC-seq. snmC-seq2 is an efficient strategy suited for large-scale single-cell epigenomic studies.

单细胞 DNA 甲基化组谱分析使人们能够研究复杂组织和细胞重编程过程中的表观基因组异质性。然而，该方法的更广泛应用受到测序文库质量不高的限制。本文报道了 snmC-seq2，与 snmC-seq 相比，该方法提供了改进的读段比对、减少了人为读段、提高了通量，以及增加了文库复杂性和覆盖均匀性。snmC-seq2 是一种适用于大规模单细胞表观基因组研究的高效策略。