Yarger J G, Armilei G, Gorman M C
Mol Cell Biol. 1986 Apr;6(4):1095-101. doi: 10.1128/mcb.6.4.1095-1101.1986.
We analyzed a cloned fragment of the yeast URA3 promoter region that contains a sequence of DNA capable of functioning as a highly efficient transcription terminator. BAL 31 deletions have shown the signal for the transcription termination activity is less than or equal to 110 base pairs and resides between bases 45 and 155 upstream of the URA3 primary ATG codon at base 227. In our in vivo assay system, the DNA fragment is able to terminate transcripts very efficiently in either orientation. The terminated transcripts bind to oligodeoxythymidylate cellulose columns and promote the synthesis of full-length cDNAs, suggesting that the transcripts are polyadenylated. The 110-base-pair region contains no sequence resembling terminator consensus sequences described by Zaret and Sherman (K.S. Zaret and F. Sherman, Cell, 28:563-573, 1982) or Henikoff and Cohen (S. Henikoff and E.H. Cohen, Mol. Cell. Biol., 4:1515-1520, 1984). We discuss the possible physiological relevance of this sequence to bona fide termination of transcription and to URA3 regulation in Saccharomyces cerevisiae.
我们分析了酵母URA3启动子区域的一个克隆片段，该片段包含一段能够作为高效转录终止子发挥作用的DNA序列。BAL 31缺失实验表明，转录终止活性信号的长度小于或等于110个碱基对，位于URA3起始ATG密码子（位于第227位碱基）上游45至155位碱基之间。在我们的体内检测系统中，该DNA片段能够在两个方向上非常有效地终止转录本。终止的转录本与寡聚脱氧胸苷酸纤维素柱结合，并促进全长cDNA的合成，这表明转录本是多聚腺苷酸化的。这个110个碱基对的区域不包含与Zaret和Sherman（K.S. Zaret和F. Sherman，《细胞》，28:563 - 573，1982年）或Henikoff和Cohen（S. Henikoff和E.H. Cohen，《分子细胞生物学》，4:1515 - 1520，1984年）所描述的终止子共有序列相似的序列。我们讨论了该序列与酿酒酵母中转录的真正终止以及URA3调控可能的生理相关性。
